流感嗜血菌及黏膜炎莫拉菌的耐药性分析  

Antimicrobial resistance of clinical isolates of Haemorphilus influenzae and Moraxella catarrhalis

作　　者：张玲[1] 程方雄[2] 杨宏伟[3] 

机构地区：[1]华中科技大学同济医学院附属普爱医院骨科,湖北武汉430033 [2]华中科技大学同济医学院附属普爱医院检验科,湖北武汉430033 [3]湖北医药学院附属太和医院检验科,湖北十堰442000

出　　处：《中华医院感染学杂志》2014年第5期1047-1048,1051,共3页Chinese Journal of Nosocomiology

基　　金：武汉市卫生局科研基金项目(WX12D14)

摘　　要：目的探讨流感嗜血菌(HIN)和黏膜炎莫拉菌(MCA)的耐药现状,为临床治疗提供参考依据。方法细菌培养和分离严格按照《全国临床检验操作规程》进行;采用法国生物梅里埃公司VITEK-2Compact全自动微生物分析仪和配套鉴定试剂盒进行菌种鉴定;采用琼脂稀释法对抗菌药物进行敏感试验,并用头孢硝噻吩纸片检测β-内酰胺酶;数据统计应用WHONET 5.5版本软件处理分析。结果喹诺酮类抗菌药物对HIN表现出较高的敏感性,左氧氟沙星和莫西沙星均为100.0%敏感,环丙沙星为99.0%;阿奇霉素的敏感率最低,为4.8%;喹诺酮类抗菌药物对MCA表现出较高的敏感性,左氧氟沙星为100.0%敏感,环丙沙星为98.2%;对阿莫西林/克拉维酸与克拉霉素保持着100.0%的抗菌活性;阿莫西林的耐药率为92.7%。结论医院务必要执行《抗菌药物临床应用管理办法》,对感染患者必须参照药敏结果结合患者具体状况抗感染治疗。OBJECTIVE To eplore the antimicrobial resistance of clinical isolates of Haemorphilus influenzae （HIN） and Moraxella catarrhalis （MCA） so as to provide the reference for clinical treatment. METHODS The bacterial culture and isolation were performed to refer to National Guide to Clinical Laboratory Procedures. then VITEK compact 2 automatic microorganism identification system Cbiolvlerieux ） was employed to identify the bacteria strains. the drug susceptibility testing to nine antibiotics was performed for by agar dilution methods. the Nitrocefin disks were used to detect β-lactamase. and the data were statistically analyzed with WHONET 5.5 software. RESULTS The HIN strains were highly susceptible to quinolones antibiotics. the drug susceptibility rates to levofloxacin and moxifloxacin were 100. 0%. ciprofloxacin 99. 0%. azithromycin 4. 8%; the MCA strains showed high susceptibility to quinolones antibiotics. the drug susceptibility rate to levofloxacin was 100. 0 %. ciprofloxacin 98. 2 %. the strains remained 100.0 % of antibacterial activity against amoxicillin-clavulanic acid and clarithromycin , and the drug resistance rate to amoxicillin was 92. 7 %. CONCLUSION It is necessary for the hospital to execute the Measures for clinical application of antibiotics and perform the anti-infection therapy according to the result of drug susceptibility testing combined with the patients specific condition.

关 键 词：流感嗜血菌属 黏膜炎莫拉菌 耐药性 

分 类 号：R378[医药卫生—病原生物学]