痰黏液型铜绿假单胞菌的分离及耐药性分析  

Isolation and resistance analysis of mucoid Pseudomonasaeruginosafrom Sputum

作　　者：李瑜珍[1] 曾学辉[1] 李忠新[1] 张春雷[1] 谢懿[1] 

机构地区：[1]深圳市中医院检验科,广东深圳518034

出　　处：《中华医院感染学杂志》2014年第5期1059-1060,1066,共3页Chinese Journal of Nosocomiology

基　　金：深圳市科技计划基金项目(201103394)

摘　　要：目的了解医院痰标本黏液型铜绿假单胞菌的分离及耐药性，为临床抗感染治疗提供依据。方法对2011年1月-2012年6月医院各病区及门诊送检合格的痰液标本1947份进行分离鉴定，采用法国生物梅里埃公司VITEK-2系统进行细菌鉴定，用K-B法进行药敏试验，采用SPSSl9．0统计软件对数据进行统计分析。结果1947份痰标本分离出铜绿假单胞菌292株，其中黏液型铜绿单胞菌60株，分离率为3．08％，占20．55％；黏液型铜绿假单胞菌对阿米卡星、哌拉西林、头孢他啶、头孢吡肟、氨曲南、美罗培南、替卡西林、哌拉西林／他唑巴坦、亚胺培南的耐药率为0～10．00％，均比非黏液型铜绿假单胞菌（17．24%～48．27％）低（P〈0．05）。结论黏液型铜绿假单胞菌体外药物敏感试验耐药性较弱，分离鉴定后结果及时报告临床，并提示临床医师这类菌株有可能形成生物膜，治疗要考虑生物膜因素。OBJECTIVE To investigate the isolation and susceptibility of mucoid Pseudomonas aeruginosa from sputum, and to provide the basis for clinical anti-infective therapy. METHODS A total of 1947 qualified sputum samples delivered from each ward and out-patient department from. 2011 to July 2012 were isolated and identified by VITEK-2 system （biolvlerieux Corporate）, and the druy resistance was determined by Kirby-Bauer method. The software SPSS19. 0 was used for statistical analysis of the obtained data. RESULTS A total of 292 stains of Pseudomonas aeruginosa were isolated from the sputum sample of 1947 patients. There were 60 strains of mucoid Pseudomonas aeruginosa （the isolate rate 3. 08 %） among all isolated Pseudomonas aeruginosa stains, accounting for 20. 55%. The resistance rate of mucoid Pseudomonas aeruginosa to Amikacin , Piperacillin , Ceftazidime , Cefepime , Aztreonarn , Meropenern , Ticarcillin , Piperacillin/Tazobactam, Imipenem was 0-10. 00%, lower than the rate 07. 24-48. 27 %） of non-mucoid Pseudomonas aeruginosa to these drugs （P〈0. 05）. CONCLUSION The in vitro drug sensitivity test showed the druy resistance senstinty of mucoid Pseudomonas was weak. The isolation and identification should be timely perfmed and reported to clinic physicians and it should be indicated to physicians that such strains are likely to form a biofilm which should be considered in the treatment.

关 键 词：黏液型铜绿假单胞菌 生物膜 耐药性 

分 类 号：R378.991[医药卫生—病原生物学]