化学发光法在感染性指标定量检测中的应用  

Implication of chemiluminescence quantitative indicators in infection detection

作　　者：黄庆华[1] 周燕[2] 吕迎霞[1] 陈楠[1] 周明锦[1] 

机构地区：[1]郑州市儿童医院核医学科,河南郑州450000 [2]郑州市妇幼保健院检验科,河南郑州450000

出　　处：《中华医院感染学杂志》2014年第5期1293-1295,共3页Chinese Journal of Nosocomiology

基　　金：高等学校博士学科点专项科研基金项目(200114407120001)

摘　　要：目的探讨化学发光法在输血前感染性指标定量检测中的临床应用,以提高检出符合率,降低漏报率。方法选择2012年1月-2013年7月医院进行手术或输血治疗的患者共2 748例,分别用化学发光法和酶联免疫吸附法输血前检测乙型肝炎表面抗原、抗丙型肝炎病毒抗体、抗苍白密螺旋体抗体、抗HIV抗体进行阳性率比较,并对化学发光法对阳性检出患者进行复检,记录复检结果一致率;化学发光法采用北京科美生物技术有限公司CHEMALIN600全自动化学发光分析仪及配套诊断试剂盒,芬兰Muitiskan MK3酶标仪和Wellwash4 MK2洗板机,英科新创(厦门)科技有限公司ELISA诊断试剂盒。结果化学发光法对各种抗体、抗原的检出率均高于酶联免疫吸附法,其中化学发光法对乙型肝炎表面抗原的检出率为12.92%,高于酶联免疫吸附法的11.21%,且两种方法之间的差异有统计学意义(P<0.05);抗丙型肝炎病毒抗体、抗苍白密螺旋体抗体、抗HIV抗体的化学发光法阳性检出率分别为1.16%、1.24%、0.07%,且与酶联免疫吸附法检出率之间的差异无统计学意义;对阳性检出患者进行复查,其中乙型肝炎表面抗原的复查一致率最低,为63.10%,且两次检查阳性率之间的差异有统计学意义(P<0.05),其他各项的复查一致率均>90.00%,且两次检查阳性率之间的差异无统计学意义。结论临床中对于感染性指标定量分析过程中应用化学发光法具有较好的应用效果,甚至优于酶联免疫吸附法,值得临床中应用。OBJECTIVE To explore the clinical usage of chemiluminescence quantitative detection of infectious indicators before transfusion, to improve the detection compliance rates and reduce false negative rate. METHODS Using chemiluminescence and enzyme-linked immunosorbent assay to detect the 2748 cases of patients pre-transfusion regarding hepatitis B surface antigen, anti-hepatitis C virus antibody, anti-Treponema palladium antibodies, anti-human immunodeficiency virus antibody positive rate for positive reexamined the positive with chemiluminescence, The consistent rate of re-examinization was recorded. RESULTS Chemiluminescence antibodies, antigen detection rate wes higher than that enzyme-linked immunosorbent assay, chemiluminescence detection rate of hepatitis B surface antigen with 12. 92 % was significantly higher than the enzyme-linked immunosorbent assay 1l. 21%, no difference compared to that with ELISA （P〈O. 05）. The detection rates of anti-hepatitis C virus antibody, anti-Treponema palladium antibodies, anti-human immunodeficiency virus antibody positive by chemiluminescence were 1. 16 %, 1. 24 %, and 0. 07 % , respectively. The re-examination was performed for positive patients, the consistentrate of re-examination was lowest （63. 10） ,the positive rate between twice detection was statistically significant difference （P 〈 0. 05） , rates of other indicator between twice checks were more than 90.00 %, and the positive rate of two checks had no significant differences. CONCLUSION Clinical Infectious indicators for quantitative analysis by chemiluminescence have a good effect, even better than the enzyme-linked immunosorbent assay, worthy of clinical application.

关 键 词：化学发光法 感染性指标 定量检测 应用 

分 类 号：R446.1[医药卫生—诊断学]