藏药湿生扁蕾对IL-6诱导SW480细胞SATA3活化作用的影响  

Influence of Gentianopsis paludosa Ma on the expression of SATA3 phosphorylation induced by IL-6 in cell line SW480

作　　者：王雅莉[1] 景明[1,2] 杨雅丽[1] 张艳霞[1] 高娜[1] 赵慧巧 刘娟娟[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

出　　处：《甘肃中医学院学报》2014年第1期10-12,共3页Journal of Gansu College of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81360522)

摘　　要：目的探讨藏药湿生扁蕾对白细胞介素-6(IL-6)诱导SW480细胞信号转录和转录激活因子(STAT3)活化作用的影响。方法选用SW480细胞为实验对象,以不同质量浓度(0,250,500,1 000μg/mL)的湿生扁蕾干预,采用Bio-plex悬液芯片法检测SW480细胞STAT3磷酸化的水平。结果不同质量浓度的湿生扁蕾均有抑制SW480细胞STAT3活化的作用,与对照组比较差异有统计意义(P<0.05),并呈质量浓度-剂量依赖关系。结论湿生扁蕾抗结肠癌的作用与其抑制IL-6介导的STAT3信号通路的活化有关。Objective To observe the effect of Gentianopsis paludosa Ma on the expression of SATA3 phosphorylation induced by IL-6 in cell line SW480. Methods SW480 K562 cells were treated at different dosages （0, 250, 500, and 1000 μg/mL） of Gentianopsis Paludosa Ma, respectively. The expression of STAT3 phosphorylation was determined by the way of bio-plex liquid chip. Results Compared with the control group, STAT3 phosphorylation was decreased significantly in SW480 cells treated with different dosages of gentianopsis paludosa ma in a time and dose dependent manner. Conclusion Gentianopsis paludosa Ma has the effect on SW480 cells may be related to the inhibition of STAT3 phosphorylation mediated induced by IL-6.

关 键 词：湿生扁蕾 SW480细胞 STAT3通路 

分 类 号：R285.5[医药卫生—中药学]