烟草正反交组合F1 SRAP的比较分析  

作　　者：朱列书[1] 詹莜国 郭东海[1] 宋正雄 王祖富[1] 尹佳[1] 

机构地区：[1]湖南农业大学中国烟草中南农业试验站,湖南长沙410128 [2]昆明市烟草公司,云南昆明650202

出　　处：《中国科技纵横》2014年第3期219-221,共3页China Science & Technology Overview

摘　　要：本文旨在利用SRAP标记对4X4完成双列杂交烟草组合进行差异比较，从遗传层面鉴定正反交差异。100对SRAP引物组合中只有me3-em2、me4-em6、me6-em5、me10-em7引物组合能4 X 4个组合中扩增出差异条带，但是没能在正反交F1之间扩增出特异条带。表明SRAP标记在鉴别种内之间的差异时效率不高。SRAP was used to compare differences of 4x4 Flue-cured Tobacco combinations by diallel crosses excluding reciprocal and identify reciprocal differences of Heredity. Amplified bands were only detected by me3-em2 prime pair、me4-em6 primer pair、me6-em5 primer pair and me10-em7 primer pair in the 100 primer pairs of 4x4 Flue-cured Tobacco combinations but specific bands were not amplified between Reciprocal Crosses Hybrids F1.The results showed that the efficiency of identification intraspecific dif erences was not high by SRAP.

关 键 词：烤烟 SRAP分子标记 差异 

分 类 号：S634.3[农业科学—蔬菜学]