甲基绿褪色分光光度法测定红霉素  

Fading Spectrophotometry Determination of Erythromycin with Methyl Green

作　　者：陈莲惠[1] 叶莉[2] 谢梦[2] 

机构地区：[1]川北医学院化学教研室,四川南充637000 [2]川北医学院药学院,四川南充637000

出　　处：《中国无机分析化学》2014年第1期76-78,共3页Chinese Journal of Inorganic Analytical Chemistry

基　　金：川北医学院重点培育项目(CBY11-A-ZP13);四川省教育厅重点项目(12ZA056)资助

摘　　要：建立了褪色分光光度法测定红霉素肠溶片中红霉素含量的方法.用二次石英蒸馏水为溶剂,甲基绿和红霉素在40℃下可以反应形成稳定的离子缔合物,以试剂空白做参比测定离子缔合物溶液的吸光度,离子缔合物的生成导致吸收光谱发生变化,且在一定范围内吸光度变化值△A与红霉素的浓度成正比,红霉素的浓度在0.000 6～0.105 0 mg/mL范围内服从Beer定律,在635 nm处测得ε=4.23×104 L/（mol· cm）-1,方法检出限达到0.26 μg/mL.方法简便快捷,重现性和选择性好,可用于红霉素肠溶片中红霉素含量的测定.We tried to develop a new method for the determination of erythromycin in enteric-coated tablets with spectrophotometry. In the neutral aqueous solution, prepared with secondary quartz distilled water as solvent, methyl green reacts with erythromycin to form ionic complex at 40 ℃. Using blank reagent as a reference, the absorbance of the ionic complex was measured. The formation of ionic complex brings some changes to the absorption spectra. Furthermore, within a certain range of concentration, the absorbance change value△A is proportional to the concentration of erythromycin. When the concentration of erythomycin is in the range of 0. 000 6-0. 105 0 mg/mL, the Beer＇s law is obeyed. The apparent molar absorptivity of the complex ε at 635 nm is 4.23× 104 L/（mol-cm） 1. The detection limit of the method is 0. 26μg/mL. The method is simple with a good reproducibility and selectivity and can be used in the determination of the content of erythromycin in erythromycin enteric-coated tablet.

关 键 词：甲基绿 红霉素 褪色分光光度法 

分 类 号：O657.32[理学—分析化学] TH744.121[理学—化学]