内毒素急性肾损伤大鼠肾组织livin的表达研究  

The expression of livin of kidney tissues in rats with acute kidney injury induced by endotoxin

作　　者：佟琳[1] 张标[2] 姚华国[1] 孙小聪[1] 陈骥[1] 观春明 

机构地区：[1]广东医学院附属医院重症医学科,广东湛江524001 [2]广东医学院组织学与胚胎学教研室,广东湛江524023

出　　处：《重庆医学》2014年第7期826-827,共2页Chongqing medicine

基　　金：广东省医学科研基金立项课题(B2009195);广东医学院青年基金(XQOT40)

摘　　要：目的探讨livin在内毒素急性肾损伤大鼠肾组织中的表达情况及其在急性肾损伤细胞凋亡中的作用。方法建立内毒素急性肾损伤大鼠模型,应用苏木精-伊红染色并检测血肌酐、尿素氮水平观察肾损伤情况;采用免疫组织化学,观察不同时间点上,内毒素急性肾损伤大鼠肾组织中livin和caspase-3的表达情况,分析其变化与肾损伤的关系。结果与对照组比较,内毒素大鼠肾组织病理形态学损伤明显,血肌酐及尿素氮水平显著升高(P<0.01),存在急性肾损伤表现。免疫组织化学检测提示,livin和caspase-3在内毒素大鼠肾组织中均有阳性表达(P<0.01),且在livin出现较高水平表达后,随时间推移,caspase-3表达增强趋势变缓。结论 livin参与了内毒素急性肾损伤的发病机制,并可能通过抑制caspase-3等重要凋亡效应蛋白,发挥减轻肾损伤、保护肾功能的作用。Objective To investigate the expression of livin of kidney tissues in rats with acute kidney injury（AKI） induced by endotoxin,and the role of livin in the cell apoptosis of AKI. Methods The rat models with AKI were induced by endotoxin. The de- gree of kidney injury was observed by hematoxylin and eosin stain,measuring the levels of the serum creatinine and urea nitrogen. The expression of livin and caspase-3 in kidney tissue at different time points was analyzed by immunohistochemistry assay,and the relationship between the expression of livin and caspase-3 and kidney injury was analyzed. Results Compared to the control group, the rats injected endotoxin had the performance of AKI, with obviously pathomorphological damage in the kidney tissues, signifi- cantly increasing in the serum creatinine and urea nitrogen（P〈0.01）. The livin and caspase-3 of kidney tissues in rats with AKI caused by endotoxin were positive expression（P〈0.01）. With the lapse of time,the trend of increasing of caspase-3 showed gradu ally slow after the highest expression of livin. Conclusion The livin involved in the pathogenesis of endotoxinqnduced AKI,it ,night relieve the kidney injury and protect renal function by inhibiting casepase 3 important apoptotic effector protein.

关 键 词：内毒素类 大鼠 Sprague—Dawley 急性肾损伤 细胞凋亡 

分 类 号：R692[医药卫生—泌尿科学]