不同剂量高压氧预处理对大鼠肝脏缺血再灌注损伤的作用  

Effects of different doses of hyperbaric oxygen preconditioning on hepatic reperfusion injury in rats

作　　者：张建军[1] 杨永丰[1] 张冰[1] 陈文林[2] 田占发[1] 王静[1] 

机构地区：[1]河北大学附属医院急诊医学科 [2]河北大学附属医院高压氧科

出　　处：《职业与健康》2014年第5期616-619,共4页Occupation and Health

摘　　要：目的观察不同剂量高压氧预处理对大鼠肝脏缺血再灌注损伤的作用,并探讨其机制。方法 40只SD大鼠随机分成4组,对照组(C组)、缺血再灌注组(I/R组)、高压氧预处理1次组(D1组)、高压氧预处理2次组(D2组)。分别对D1和D2组大鼠进行高压氧预处理,连续10日后建立肝脏缺血再灌注损伤模型,检测各组血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)以及组织中Na+-K+ATP酶的活力。光学显微镜下观察各组大鼠肝脏组织结构的变化。结果与对照组相比,I/R组大鼠肝功能损害明显;D1组大鼠ALT和AST活力较I/R组显著降低,SOD和组织中Na+-K+ATP酶活力显著升高;D2组大鼠ALT和AST活力较I/R组显著升高,SOD和组织中Na+-K+ATP酶活力显著降低。光学显微镜下D1组肝细胞轻度水肿,嗜酸性变减轻,肝窦内轻度瘀血;D2组肝脏结构紊乱,肝细胞片状水肿,可见大量空泡状,灶状嗜酸性变,肝窦瘀血明显。结论适度剂量的高压氧预处理可减轻大鼠缺血再灌注肝脏损伤,而过高剂量的高压氧预处理可加重肝脏损伤。[ Objective ] To study the effects of different doses of hyperbaric oxygen preconditioning on hepatic reperfusion injury in rats, and investigate the mechanism. [ Methods] Forty SD rats were divided into 4 groups randomly, control group （C group）, is- chemia reperfusion group （I/R group}, HBO preconditioning 1 time group （D1 group）, HBO preconditioning 2 times group （D2 group）. The vascular pedicle of the liver was obstructed to establish liver I/R model after the rats pretreated with hyperbaric oxygen in D1 group and D2 group for 10 days. Plasma alanine aminotransferase （ALT） , aspartate aminotransferase （AST） , superoxide dismutase （SOD） and liver tissue Na ＋ -K ＋ ATPase of each group were measured. The structure of liver was observed by light mi- croscopy. [ Results] Compared with the control group, the hepatic injury in rats were obvious in I/R group; ALT and AST level in group D1 were decreased significantly than group I/R, SOD level and Na ＋ -K＋ ATPase in liver organization in group D1 were in creased significantly than group I/R; ALT and AST in group D2 were increased significantly than group I/R,SOD and Na ＋ -K ＋ ATPase in group D2 were decreased significantly than group I/R. Under microscope, mild edema to hepatocytes, eosinophilic variant miti- gation, mild bleeding in the hepatic sinusoid were observed in group D1 rats; liver structural disorder, hepatocellular flake edema, spreading vacuoles in the cytosol and extracellular space, focal eosinophilic variant and blood congestion in the hepatic sinusoid were observed in D2 group. [ Conclusion] Moderate doses of the hyperbaric oxygen can protect the rats liver against ischemia-reper- fusion injury, but higher doses of the hyperbaric oxygen may aggravate hepatic injury.

关 键 词：高压氧 缺血再灌注损伤 预处理 肝脏 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]