激光微束穿刺法获得抗虫转基因油菜及其后代的研究  

Acquisition of insect-resistant transgenic Brassica napus and Its Progeny by laser microbeam puncture

作　　者：侯丙凯[1] 周奕华[1] 刘桂珍[1] 宋桂英[1] 王兰岚[1] 陈正华[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《中国激光》2001年第1期93-96,共4页Chinese Journal of Lasers

摘　　要：利用激光微束穿刺法将苏云金芽孢杆菌的毒蛋白 (δ endotoxin)基因导入了油菜 ,经聚合酶催化链式反应(PCR)和PCRSouthern杂交证明抗虫基因已导入油菜基因组中并能在T1 代得到遗传。对转基因植株进行了抗虫性测试 ,结果表明某些植株具有较好的抗虫性 ,并且这种抗虫性在T1 代仍能保持。实验结果表明抗虫基因已整合进油菜基因组并得到了稳定的遗传。The introduction of insect resistant δ endotoxin gene from Bacillus thuringiensis into Brassica napus was carried out by laser microbeam puncture. The acquisition of transgenic plants and the inheritance of transgene in T 1 generation were confirmed by PCR and PCR Southern blot hybridization. The bioassay of transgenic plants showed that some of the plants exhibited tolerant to pest insects and insect resistance was maintained in the T 1 generation. These results showed that the insect resistance gene was integrated into the genome of Brassica napus and was stably inherited.

关 键 词：激光微束穿刺 抗虫基因 油菜 转基因植株 

分 类 号：S565.4[农业科学—作物学]