并联式双梯度反相离子对液相色谱法快速检测土壤中芳磺酸  

The Rapid Detection of Seven Kinds of Aromatic Sulfonic Acid in Soil by Parallel and Dual Ion-Pair RP-HPLC Gradient Elution Method

作　　者：杨柳[1] 马浩[1] 李桂香 贺伟[1] 施超欧[1] 

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]澳实分析检测(上海)有限公司,上海201206

出　　处：《中国环境监测》2014年第1期159-163,共5页Environmental Monitoring in China

摘　　要：通过阀切换并联式双系统,建立了一套测定土壤样品中7种芳磺酸的反相离子对液相色谱法。该方法采用2根色谱柱,一根色谱柱分析的同时,另一根色谱柱通过阀切换实现平衡,节约了38.5%的分析时间。方法使用Dikma Diamonsil C18(150 mm×4.6 mm×5μm)色谱柱,以离子对试剂(四丁基硫酸氢铵)和乙腈为流动相,检测波长为226、235、258 nm。各组分线性关系良好,线性相关系数均在0.999 9以上。随机抽取样品进行重新提取、检测,得到各组分的相对标准偏差均小于7%,且定量限最高不超过0.181 2μg/g,重复性和定量限均能满足实验要求。Established a method for extraction and fast detection of 7 kinds of aromatic sulfonic acid （ anthraquinone 1, 5-disulfonic acid, 1-amino-8-naphthol-3, 6-disulfonic acid, 1-naphthol-4-sulfonic, anthraquinone-1, 8-disulfonic acid, 1-naphthalene sulfonic acid, 2-naphthalene sulfonic acid, 3-hydroxy-2-naphthoic acid） in soil by valve switching Ion-pair RP-HPLC with a dual system. Two columns were used in this system. When one was in the process of determination, the other one was in the process of equilibration. Thus 38.5% time was saved. Analytical columns were Dikma Diamonsil C18 （150 mm ×4.6 mm ×5μm） , eluents were ion-pair reagent （ Tetrabutylammonium Hydrogen Sulfate ） and acetonitrile, and wave lengths were 226, 235, 258 nm. A good linear relationship was obtained for each aromatic sulfonic acid. All linear correlation coefficients were more than 0.999 9. Extracted and detected the randomly selected samples, and got each RSD of the 7 compounds less than 7% as well as the quantification limit no more than 0.181 2 μg/g. Both of the repeatability and quantification limit meet the requirements of detection.

关 键 词：芳磺酸 阀切换 双系统 反相离子对液相色谱 

分 类 号：X830.2[环境科学与工程—环境工程] O657.72[理学—分析化学]