紫外光谱法研究药物与DNA作用方式——药物对DNA碱变性的影响  

UV STUDY ON THE WAYS OF INTERACTIONS OF DRUGS WITH DNA——EFFECTS OF CDDP, Cp_2TiCl_2 AND DHE ON ALKALI DENATURATION OF DNA

作　　者：丁道远[1] 胡皆汉[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116012

出　　处：《光谱学与光谱分析》1991年第6期7-10,共4页Spectroscopy and Spectral Analysis

摘　　要：抗癌药顺铂(CDDP)的抗癌机理研究表明,DNA是顺铂进攻的靶子,并且通过与DNA中鸟嘌呤中N7配位,通过形成链内交联作用使DNA功能受损。二氯二茂钛也具有抗癌活性,并且它作用的靶子也是DNA。我们曾报道了它能使DNA—EB的荧光强度降低。目前有关它与DNA作用的细节仍很不清楚。β-榄香烯是中药莪术中抗癌的有效成分,由于它只含有C和H两种元素而引起了人们的重视。曾有报道,Based on the measurement of A_(260) in different pH values, the alkali denaturation curves of calf-thymus DNA were obtained before and after the addition of drugs (CDDP, Cp_2TiCl_2 and two-hydroxy derivative of β-elemene DHE). The results show that the stability and hyperchromaticity of DNA decrease after the addition of of CDDP, because CDDP interacts with DNA through inter-, intra-strand cross-linkings. For Cp_2TiCl_2 the stability and hyperchromaticity are not changed, therefore there are no cross-linkings. So, the mechanism of interaction of Cp_2TiCl_2 with DNA is greatly different from thai of CDDP. For DHE, there is no change for hyperchromaticity, but the stability of DNA decreases a little. Because of no crosslinkings, the reason of the decrease is different from that of CDDP. However, from the decrease, we may get a conclusion that DHE does not interact with DNA through intercalation.

关 键 词：药物 脱氧核糖核酸 紫外光谱法 

分 类 号：R966[医药卫生—药理学]