大孔树脂纯化葛根的使用次数和再生方法  

Life and intensified regeneration technology of macroporous resins in extracting and purifying puerarin and total flavonoids from pueraria lobata

作　　者：刘慧敏[1] 杜守颖[1] 陆洋[1] 李鹏跃[1] 冯健男[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《天津中医药》2014年第3期177-180,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81073057);北京中医药大学复方中药制剂研究创新团队发展计划资助(2011-CXTD-13)

摘　　要：[目的]研究HPD-200A型大孔树脂在纯化葛根素及葛根总黄酮时的使用寿命和再生方法。[方法]以葛根总黄酮和葛根素的吸附容量和精致后的纯度为指标,考察大孔树脂在多次吸附洗脱和再生后的吸附洗脱能力的变化。[结果]树脂初次使用时,葛根素吸附容量约为17.29 g/L,总黄酮的吸附容量约为46.00 g/L,两者的纯度为32.40%和75.52%,连续使用11次后葛根素吸附容量为原来的82.26%,总黄酮的吸附容量为原来的80.99%,两者纯度为27.23%和67.99%,纯度略有降低。用95%乙醇再生处理后,HPD-200A大孔树脂对葛根素和总黄酮的吸附容量分别可达到新树脂的94.35%和91.87%,两者的纯度也均可达到新树脂的97%以上。[结论]使用HPD-200A型大孔树脂精制葛根时,新树脂可连续使用11次,用95%乙醇再生后,至少可连续使用3次,即一批HPD-200A型树脂至少可使用14次,本研究为葛根纯化工艺的产业化确定提供了实验基础和参考。[Objective] To study the life and intensified regeneration technology of HPD-200A macroporous resins in purifying puerarin and puerarin flavonoids. [Methods] With the adsorption capacity and purity of puerarin and total flavonoids as indexes, the absorption capacity of HPD-200A macroporous resins which were multiple absorbed and intensified regenerated was studied. [Results] First, the adsorption capacity of puerarin and total flavonoids was about 17.29 g/L and 46.00 g/L, respectively. Their adsorption capacity became 82.26% and 80.99% of the original and the purity was 27.23% and 67.99% after HPD-200A maeroporous resin was used continuously for 11 times. With 95% glycol regeneration, the adsorption capacity of HPD-200A macroporous resins for puerarin and total flavonoids could reach above 94.35% and 91.87%, the purity was more than 97% of the new resin. [Conclusion] With HPD-200A macroporous resin refining puerarin, new resin can be used for 11 times continuously. When regenerated with 95% ethanol, it can be used at least three times again, that is, the HPD-200A resin can be used at least for 14 times.

关 键 词：大孔树脂 葛根 总黄酮 再生 

分 类 号：R285.5[医药卫生—中药学]