UPLC-MS／MS法测定吸毒者头发中10种毒品代谢物的含量  

UPLC-MS/MS determination of metabolites for 10 drugs resided in the hair of drug abusers

作　　者：谢普[1,2] 王铁杰[2] 殷果[2] 闫研[2] 丁振浩[2] 肖丽和[2] 詹华强[4] 梁嘉荣[4] 李璐[1,2] 孙新珺 毕开顺[1] 

机构地区：[1]沈阳药科大学,沈阳110016 [2]深圳市药品检验所,深圳518057 [3]深圳市公安局强制隔离戒毒所,深圳518019 [4]香港科技大学中药研发中心,中国香港

出　　处：《药物分析杂志》2014年第3期516-522,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立快速、准确的UPLC—MS／MS方法测定人头发中残留的海洛因、冰毒、摇头丸、苯环己哌啶、美沙酮、可尤因、氯胺酮等10种毒品。方法：头发样品经清洗、剪碎。甲醇-乙腈-2mmol·L^-1甲酸铵溶液（25：25：50），超声提取，5种同位素内标物，采用ZorbaxECLIPSEPlusC18（2．1mm×50mm，1．8μm）色谱柱，0．1％甲酸乙腈-0．1％甲酸水为流动相，流速0．35mL·min^-1梯度洗脱，质谱采用ESI＋离子源，MRM监测模式，检测6一乙酰基吗啡、吗啡、可待因、甲基安非他明、亚甲二氧基甲基苯丙胺、亚甲二氧基乙基苯丙胺、亚甲二氧基苯丙胺、苯环己哌啶、美沙酮、苯甲酰爱康宁、可卡因、去甲可卡因、古柯乙烯、去甲氯胺桐、氯胺酮、6-乙酰基吗啡，16种化合物的定量离子对和定性离子对。结果：16种化合物在2-1000ng·mL^-1浓度范围内线性良好，日间和日内精密度RSD均小于14．6％，回收率在83．3％～110％。结论：本方法可用于吸毒者头发中残留的10种毒品代谢物的含量测定。Objective：To develop a rapid and accurate method for the determination of 10 drugs resided in human hair by UPLC - MS/MS, whith were heroine,ice, ecstasy, pcp, metadone, cocaine, ketamine, et al. Method： Hair sam- ples were decontaminated and cut,followed by ultrasonic extraction with a solution consisted of methanol ：acetonitrile： 2 mmol·L^-1 ammonium formate solution （25： 25： 50）. Using 5 kinds of isotope standard as the internal standard, the samples were separated on a Zorbax ECLIPSE Plus Cl8 （2. 1 mm × 50 mm, 1.8 μm）column,eluted at the flow rate of 0. 35 mL ·min^-1 by 0. 1% formic acid - acetonitrile and 0. 1% formic acid - water. MS instrument equipped with ESI＋ ion source and MRM mode was used to detect the quantitative and qualitative ion pairs of 16 compounds,whith were 6 - acetylmorphine, morphine, codeine, methamphetamine, amphetamine, MDMA, MDA, phencyclidine, metha- done,benzoylecgonine, cocaine, norcocaine, cocaethylene, norkeamine, ketamine, 6 - acetylmorphine - D6. Results： The 16 compounds showed a good linearity in the concentration range of 2 - 1000 ng·mL^-1, RSD of the intra - and inter- day precision was less than 14. 6% ,and the recovery was in the range of 83.3% -110%. Conclusion： This method is suitable for the quantitative determination of the metabolites of 10 drugs in the hair of drug abusers.

关 键 词：头发样品 毒品检测 代谢物含量 超高效液相串联质谱 海洛因 冰毒 摇头丸 苯环己哌啶 美沙酮 可卡因 氯胺酮 

分 类 号：R917[医药卫生—药物分析学]