苯基甲巯咪唑对内毒素休克小鼠的保护作用  

Protective effect of phenylmethimazole on endotoxic shock mice

作　　者：罗毅[1] 刘明祥[1] 黄振宇[1] 刘路培[1] 毛文雄[1] 黄善华[1] 龙春萍[1] 

机构地区：[1]柳州市人民医院急诊科,广西柳州545006

出　　处：《中华医院感染学杂志》2014年第6期1304-1306,1310,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(59637050)

摘　　要：目的探讨苯基甲巯咪唑(C10)注射对内毒素休克小鼠生存改善效应的量效关系,以及不同给药时间的影响。方法小鼠随机分为C10预处理不同剂量组(0.25、0.5、1.0mg/kg)、生理盐水对照组、基线对照组以及C10后处理组(造模后30min注射),采用脂多糖(LPS,25mg/kg)腹腔内注射造模,在10个不同时间点观察小鼠的生存情况并作中毒症状评分(ESS),观察终点存活鼠和提前死亡鼠均留取肺、肾、肝组织行HE染色和组织病理观察,使用STATA10.0软件对实验数据进行统计分析。结果造模后12hC10预处理组和C10后处理组分别与生理盐水组的ESS比较,差异均有统计学意义;C10预处理组、生理盐水对照组以及C10后处理组7d生存率分别为0、25.0%、75.0%、0、62.5%;1.0mg/kg C10预处理组和1.0mg/kg C10后处理组生存曲线比较差异无统计学意义;死亡鼠靶器官病理学检查可见显著炎性渗出和组织损伤。结论 C10预处理对内毒素休克小鼠的保护作用存在明显的量效关系;同等剂量C10在造模前后给药对内毒素休克小鼠生存具有同等的保护效应。OBJECTIVE To explore the influence of dosage and intervention time of phenylmethimazole （C10） on the survival of endotoxic shock （ES） mice. METHODS The mice were randomly divided ir%to three dosages C10 pretreatment groups （including 0.25 mg/kg, 0.5 mg/kg, and 1.0 mg/kg）, normal saline group, baseline control group, and C10 post-treatment group （ injection at 30 rain after modeling）, lipopolysaccharide （LPS, 25mg/kg） was injected for modeling in the abdominal cavity, the survival status of the mice was observed respectively at 10 time points,endotoxic shock score （ESS） was used to evaluate the symptoms of ES mice; HE staining and optical microscopes were used to observe pathological manifestation in lung, kidney, and liver of all the mice, and the experimental data were statistically analyzed with the use of STATA10.0 software. RESULTS Twelve hours after the modeling, the difference in the ESS between the C10 pretreatment group, C10 posttreatment group, and the normal saline group was statistically significant. The 7 day survival rates of the 3 C10 pretreatment groups （0.25 mg/kg,0. 5mg/kg, lmg/kg）, the control group and the C10 post-process group came respectively as the following： 0, 25%, 75%, 0%, and 62.5%. No statistical difference manifested between the C10 pretreatment group and the C10 posttreatment group at the dosage of 1.0mg/kg. Inflammatory infiltration and tissue injuries were witnessed in the targeted organs of the dead mice through histopathological examination. CONCLUSION There is obvious dose-effect relationship between C10 pretreatment ,and the survival of ES mice; a similar protection exists in the C10 pretreatment group and C10 post-process group at the same dosage.

关 键 词：苯基甲巯咪唑 脓毒症小鼠 生存率 内毒素 量效关系 脂多糖 

分 类 号：R978.1[医药卫生—药品]