两种植物生长调节剂对洋桔梗离体培养的效应  

Effect of Two Plant Growth Regulators on in vitro Culture of Eustoma grandiflorum

作　　者：孙慧晶[1] 李建宾[2] 希从芳[2] 王俊超[2] 薛英利 王有国[1] 

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2014年第2期208-215,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省自然科学基金项目(2009CD054);云南省对外科技合作计划(2013IA036)

摘　　要：研究了6-BA和NAA诱导洋桔梗外植体再生植株的效应.结果表明,培养基MS＋ 6-BA 0.1mg/L＋ NAA 0.05mg/L适于诱导洋桔梗外植体产生愈伤组织,MS＋ 6-BA 0.2 mg/L＋ NAA 0.1 mg/L适合愈伤组织的继代培养;随6-BA浓度的增加,不定芽的增殖率和玻璃化率提高,适于不定芽分化和增殖的培养基为MS＋ 6-BA 0.5mg/L＋ NAA0.1 mg/L,生根适宜的培养基为1/2 MS＋NAA 0.1～0.3 mg/L.因此,不同浓度的6-BA和NAA组合对洋桔梗茎、叶、芽等外植体愈伤组织和不定芽的诱导与增殖效果不同.试验结果为洋桔梗健康种苗生产奠定了基础.The leaves,stems,axillary bubs explants from Eustoma grandiflorum were cultured on MS medium supplemented with 6-BA and NAA of diffferent concentrations to induce regeneration plants.The results indicated that the effects of 6-BA and NAA combinations of different densities on the differentiation and proliferation of the callus and uncertain roots of the leaves,stems and bubs were different.The suitable culture medium for callus differentiation of E.grandiflorum was MS ＋ 6-BA 0.1 mg/L ＋ NAA 0.05 mg/L and for callus proliferation was MS ＋ 6-BA 0.2 mg/L ＋ NAA 0.1 mg/L.The proliferation multiples and vitrification rate of uncertain bud were improved with increasing the density of 6-BA,the suitable culture medium for differentiation and proliferation of uncertain buds was MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.1 mg/L and for root formation was 1/2 MS ＋ NAA 0.1 ～0.3 mg/L.These results established a basis for healthy seedling production of E.grandiflorum species and hybrids.

关 键 词：洋桔梗 组织培养 植物生长调节剂 茎段 腋芽 

分 类 号：S482[农业科学—农药学] S681.9[农业科学—植物保护]