聚乙二醇干扰素α-2a对慢性乙型肝炎患者树突状细胞B7-H1表达的影响  

Dynamic Changes of the Expression of B7-H1 on DCs in Chronic Hepatitis B Patients Treated with PEG-IFN alpha-2a

作　　者：汪云[1] 汪佳欣 李红霞[1] 梅夏齐 王媛媛[1] 宋波[1] 梁琦[2] 

机构地区：[1]哈尔滨医科大学附属第四医院感染科,黑龙江哈尔滨150001 [2]黑龙江省疾病控制中心,黑龙江哈尔滨150036

出　　处：《现代生物医学进展》2014年第8期1485-1489,共5页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金项目(D201076);黑龙江省教育厅基金项目(11551250)

摘　　要：目的:观察聚乙二醇干扰素α-2a对慢性乙型肝炎患者外周血树突状细胞功能及B7-H1的影响,探讨慢性乙型肝炎病毒逃逸的的机制。方法:慢性乙型肝炎患者31例,给予聚乙二醇干扰素α-2a180μg抗病毒治疗52周,分别于0、12、26、52周检测肝功能、HBV-DNA;流式细胞术检测外周血mDC表面HLA-DR、CD80、CD86、CD83、CD1a、B7-H1水平。根据患者HBV-DNA水平,将患者分为应答组(A组)、非应答组(B组),10例健康志愿者作正常对照组(C组)。结果:慢性乙肝患者的树突状细胞膜表面分子HLA-DR、CD80、CD86、CD83、CD1a的表达均降低。聚乙二醇干扰素α-2a治疗后应答组膜表面分子HLA-DR、CD80、CD86、CD83、CD1a的表达高于非应答组65.3±6.2%vs 44.2±5.5%,67.2±7.4%vs 37.3±7.2%,68.4±3.6%vs 42.5±7.3%,65.6±6.8%vs 43.2±3.9%,49.4±9.5%vs 37.5±7.9%,(P<0.05)。应答组B7-H1表达水平较治疗前下降,非应答组B7-H1水平无明显变化12.73±3.8%vs 25.24±2.92%,(P<0.05)。结论:慢性乙型肝炎患者树突状细胞功能低下,聚乙二醇干扰素α-2a治疗可以提高树突状细胞功能,降低B7-H1表达,促进HBV-DNA的清除。树突状细胞功能低下及B7-H1高表达是乙型肝炎病毒免疫逃逸的因素之一。Objective： To study the dynamic changes of the expression of B7-H1 on dendritic cells （DCs） in chronic hepatitis B （CHB） patients undergoing PEG-IFN alpha-2a therapy. Methods： 31 patients with chronic hepatitis B were given with PEG-IFN alpha-2a 180 Ixg, once a week for 52 weeks. Hepatic function and HBV-DNA were detected at week of 0, 12, 26, 52 respectively. The expression of HLA-DR, CD80, CD86, CD83, CDla, B7-H1 on DCs were detected by flow cytometry. According to HBV-DNA levels, the patients were divided into responding group （A）, non-responding group （group B）, 10 healthy volunteers as control group （group C）. Results： The expression of HLA-DR, CD80, CD86, CD83, CDla on DCs in patients with CHB were lower than control group （P〈0.05）. After PEG-IFN alpha-2a therapy, the expression of HLA-DR, CD80, CD86, CD83, CDla on DCs upregulated in responding group than non-responding group （65.3± 6.2 % vs 44.2± 5.5 %, 67.2± 7.4% vs 37.3± 7.2 %, 68.4± 3.6 % vs 42.5± 7.3 %, 65.6± 6.8 % vs 43.2± 3.9 %, 49.4± 9.5 % vs 37.5± 7.9 %, （P〈0.05））. B7-H1 expression on DCs were persistently decreased in the responding group after PEGIFN α-2a treatment, while nonresponding group maintained high level of B7-H1 expression （12.73± 3.8 % vs 25.24± 2.92 %,（P 〈0. 05））. Conclusion： PEG-IFNα-2a therapy can improve the function of dendritic cells. The expression of B7-H1 on DCs was down-regulated with HBV-DNA clearance after PEGIFN α-2a treatment. Dendritic cell dysfunction indicated by the increased B7-H1 expression is one of the factors of hepatitis B virus immune escape.

关 键 词：聚乙二醇干扰素Α-2A 慢性乙型肝炎 树突状细胞功能 B7-H1 HBV-DNA 

分 类 号：R512.62[医药卫生—内科学]