周期性张应力刺激下成肌细胞钙网蛋白的表达及变化  

Expression and Change of CRT in Stretch-induced Apoptosis of Myoblast

作　　者：李建平[1] 王洪玲[2] 高玉丽[3] 丁弦[1] 夏晨蕾 贾萍萍[1] 张强[1] 王爽玉[1] 阎潇[1] 刘文[1] 张月[1] 姚如永[4] 袁晓[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院口腔医学中心,山东青岛266075 [2]泰安市口腔医院,山东泰安271000 [3]青岛大学附属医院,山东青岛266555 [4]青岛大学医学院附属医院中心实验室,山东青岛266003

出　　处：《现代生物医学进展》2014年第9期1657-1661,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31170891)

摘　　要：目的:检测成肌细胞钙网蛋白(CRT)在循环拉伸应力刺激下的表达变化。方法:体外构建面颌部成肌细胞力学刺激模型。加力组以0.5赫兹的加载频率和10%细胞拉伸变形幅度对细胞进行加力培养1 h,6 h,12 h,24 h,运用实时荧光定量PCR技术跟Western Blot技术分别检测在周期性张应力作用下成肌细胞CRT在基因水平及蛋白水平的表达变化。结果:当对细胞加力6 h后,CRT mRNA及蛋白表达量开始增多,加力12 h后CRT mRNA及蛋白表达量到达最多(P<0.01),加力0h组与加力12 h组之间差异有显著的统计学意义(P<0.01)。结论:持续的周期性张应力刺激下CRT mRNA及蛋白表达增加。Objective： To investigate the changes of Calcium protein （CRT） expression in myoblast under cyclic tensile stress stimulus. Methods： The culture-tensile stimulate model of myoblast in vitro was established. The strength group cells were cultured for 1 h, 6 h, 12 h, 24 h under 0.5 hz loading frequency and 10% tensile deformation amplitude. PCR and Western Blot technique was used to detect the changes of CRT in the genetic level and protein level under cyclic tensile stress. Results： After 6 h cyclic tensile stress, the expression ofCRT mRNA and protein began to increase, after 12 h cyclic tensile stress, the expression ofCRT mRNA and protein reached a maximum （P〈0.01）, the difference between 0 h group and 12 h has obvious statistical significance （P〈0.01）. Conclusions： The expression of CRT mRNA and protein can increase under continuous tensile stress stimulation.

关 键 词：张应力 钙网蛋白 细胞凋亡 功能矫形 成肌细胞 

分 类 号：R318.01[医药卫生—生物医学工程] R783.5[医药卫生—基础医学]