商陆皂苷甲致肾细胞毒性的研究  

Study on Renal Cell Toxicity Induced by Esculentoside A

作　　者：周倩[1] 姚广涛[1] 金若敏[1] 谢家骏[1] 

机构地区：[1]上海中医药大学药物安全评价研究中心,上海201203

出　　处：《世界中医药》2014年第2期151-154,共4页World Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(编号:2009CB522807);国家"十二五"科技重大专项课题"中药安全评价技术平台"(编号:2011ZX09301-009)

摘　　要：目的:考察商陆皂苷甲Esculentoside A(EsA)对人肾小管上皮细胞(HK-2细胞)的毒性。方法:1)MTT法测细胞活力;2)检测细胞上清液LDH、细胞内SOD及MDA;3)采用倒置显微镜及电镜观察细胞形态学;4)采用Hoechst-PI染色及流式细胞仪观察细胞凋亡的情况。结果:1)EsA对肾细胞的活力有显著的抑制作用,其IC50为149.11μg·mL-1,且存在一定的时效和量效关系;2)EsA能升高肾细胞培养上清中的LDH,使肾细胞内SOD/MDA比值有所下降;3)EsA能使肾细胞及其超微结构发生变化,出现凋亡或坏死,有一定的量效关系。结论:大于100μg·mL-1浓度的商陆皂苷甲对HK-2细胞有一定的毒性,其毒性机制与细胞氧化应激、凋亡等相关。Objective：To investigate the toxicity of Esculentoside A on HK-2 cells （Human renal tubular epithelial cells ）.Methods：1） MTT assay was used to test the cell viability .2）The LDH in cells supernatant, SOD and MDA in the cells were detected .3）Morphologi-cal changes of the cells were observed through inverted phase contrast microscope and electron microscopy .4） Cell apoptosis was detec-ted by Hochest33342-PI dyeing and flow cytometry .Results：1） Esculentoside A could inhibit nephrocyte viability dependently on time and dose （IC50 was 149.11μg· mL-1）.2）Esculentoside A could increase the activity of LDH in nephrocyte supernatant , reduce SOD activity and increase MDA content in nephrocytes .3 ） Esculentoside A could cause damage of nephrocyte structure , cell apoptosis and necrosis.Conclusion：It showed that large dose of Esculentoside A （more than 100 μg· mL-1） has toxicity on HK-2 cells and the mechanisms of toxicity may include cellular oxidative damage and cell apoptosis .

关 键 词：商陆皂苷甲 HK-2细胞 肾细胞毒性 

分 类 号：R285.5[医药卫生—中药学]