参蛇软膏的质量标准研究  

Study on the quality standard of Shenshe Ointment

作　　者：石小鹏[1] 缪珊[2] 李捷[2] 毕琳琳[2] 苗青[2] 栗艳[1] 

机构地区：[1]第四军医大学西京医院药剂科,西安710032 [2]第四军医大学药学系天然药物学教研室,西安710032

出　　处：《西北药学杂志》2014年第2期114-117,共4页Northwest Pharmaceutical Journal

基　　金："重大新药创制"科技重大专项项目(编号:2010ZXJ09105-07B);陕西省社会发展攻关计划(编号:2012K14-05-04)

摘　　要：目的：建立参蛇软膏的质量标准。方法采用薄层色谱法对方中的苦参、黄柏、蛇床子和土茯苓进行定性鉴别；采用高效液相色谱法对制剂中蛇床子素进行定量分析。结果薄层色谱鉴别分离效果好，专属性强；蛇床子素质量浓度在17.6～88.0 mg· L -1范围内线性关系良好，r＝0．9999；平均加样回收率为99．6％，RSD为0．4％。结论该方法简单、准确、专属性强、重复性好，可有效控制参蛇软膏的质量。Objective To establish the quality standard of Shenshe Ointment .Methods Radix Sophorae Flavescentis ,Cortex Phel-lodendri Chinensis ,Fructus Cnidii ,and Rhizoma Smilacis Glabrae in Shenshe Ointment were identified by TLC .The content of osthole was determined by HPLC .Results The TLC identification was distinct and specific .The linear range was 17 .6-88 .0 mg·L -1 with good correlation coefficient （r=0 .999 9） .The average recovery was 99 .6% and RSD was 0 .4% .Conclusion The established methods are accurate ,exclusive ,reproducible and suitable for the quality control of Shenshe Ointment .

关 键 词：参蛇软膏 蛇床子素 质量标准 薄层色谱 

分 类 号：R927.11[医药卫生—药学]