磁性复合粒子固定化葡萄糖氧化酶的制备  

Preparation of immobilized glucose oxidase on magnetic composite particles

作　　者：白刚[1,2] 刘艳春[1,2] 李凤艳[3] 钱红飞[1,2] 

机构地区：[1]绍兴文理学院纺织服装学院,浙江绍兴312000 [2]浙江省清洁染整技术研究重点实验室,浙江绍兴312000 [3]天津工业大学纺织学部,天津300387

出　　处：《纺织学报》2014年第3期62-67,共6页Journal of Textile Research

基　　金：浙江省自然科学基金资助项目(LY12C20010)

摘　　要：采用化学共沉淀法制备了Fe3O4粒子,并将带正电荷的PDDA吸附在其表面,形成PDDA-Fe3O4磁性复合体。为提高葡萄糖氧化酶的稳定性和重复利用率,以自制的PDDA-Fe3O4磁性复合体为载体,通过静电引力来固定葡萄糖氧化酶。结果表明:PDDA-Fe3O4磁性载体表面的Zeta电位为47.6 mV,载体平均粒径为275 nm。葡萄糖氧化酶固定化最佳条件为:温度15℃,pH=6,酶与载体质量比为1∶66。固定化酶的热稳定性、pH值稳定性及重复使用性能都有很大的提高。Magnetic Fe3O4 was synthesized by chemical coprecipitation. PDDA with positive charge was adsorbed on the surface of F%O4 , forming PDDA-Fe3O4 composites. In order to improve the stability and repeating utilization factor of glucose oxidase, glucose oxidase was immobilized on the self-made magnetic PDDA-Fe3O4 carrier via electrostatic interaction. The results showed that the average size and Zeta potential of PDDA-Fe3O4 particle were 275 nm and 47.6 mV, respectively. The optimal immobilization conditions were as follows： temperature 15 ℃ ,pH =6 ,and the mass ratio of enzyme and carrier of 1： 66. The thermal stability, pH stability and operational stability of the immobilized enzyme were all improved greatly.

关 键 词：磁性载体 葡萄糖氧化酶 固定化 性能 

分 类 号：TQ314.1[化学工程—高聚物工业]