溴甲酚绿探针共振瑞利散射法测定大观霉素  

Resonance rayleigh scattering spectra method for the determination of spectinomycin with bromocresol green as probe

作　　者：庞向东[1] 徐艳[1] 江虹[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《中国抗生素杂志》2014年第3期214-216,共3页Chinese Journal of Antibiotics

基　　金：重庆市教委科技基金资助项目(No:KJ121321)

摘　　要：目的建立测定痕量大观霉素的共振瑞利散射法。方法在pH9．68的Tris-盐酸缓冲介质中，溴甲酚绿与大观霉素相互作用后共振瑞利散射显著增强，在340nm处，增强的共振光散射强度△IRRS与一定抟度范围的大观霉素线性相关。结果在340nm处，大观霉素的浓度在1．0×10^-7～1．0×10^-6mol／L范围内与△IRRS成正比，方法的检出限（3Sb／s）为0．030mg／L。结论该法用于市售大观霉素药物及尿样中大观霉素含量的测定，结果满意。Objective To develop a novel resonance rayleigh scattering method for determination of trace spectinomycin. Methods In pH9.68 Tris-HCl buffer medium, the resonance rayleigh scattering intensity of bromocresol green was greatly enhanced when spectinomycin was added. And the strongest △IRRS was located at 340nm. The scattering intensity（△IRRs）was directly proportional to the definite quality concentrations of spectinomycin. Results The intensity of RRS was in proportion to the concentration of spectinomycin in the range of 1.0×10^-7～1.0×10^-6mol/L, with a determination limit（3S/S）was 0.030mg/L. Conclusion The method has been applied to the determination ofspectinomycin in the urine and commercially available spectinomycin medicament with satisfactory results.

关 键 词：溴甲酚绿 大观霉素 共振瑞利散射 

分 类 号：R978.1[医药卫生—药品] R917[医药卫生—药学]