鲍曼不动杆菌耐药性及ArmA甲基化酶检测与分析  

Detection and analysis of drug-resistance and ArmA methylase of Acinetobactor

作　　者：黄和赞 罗来恒 万鸣春 杨志兰[2] 

机构地区：[1]南昌市中西医结合医院,南昌330003 [2]南昌大学第二附属医院,南昌330006

出　　处：《中国抗生素杂志》2014年第3期234-235,I0003,共3页Chinese Journal of Antibiotics

基　　金：南昌市科技计划项目([2009]17114)

摘　　要：目的了解鲍曼不动杆菌耐药性及ArmA甲基化酶携带状况,为临床治疗和防控医院感染提供依据。方法临床标本中分离97株鲍曼不动杆菌,经VITEK 2 Compact细菌仪鉴定和药敏试验,筛选氨基糖苷类耐药鲍曼不动杆菌,再采用PCR检测ArmA甲基化酶并序列分析。结果头孢菌素类、氨基糖苷类、喹诺酮类三类以上抗菌药物耐药率50.3%,氨基糖苷类耐药鲍曼不动杆菌ArmA甲基化酶检出率89.6%(43/48),有5株表型耐药未检出ArmA甲基化酶。结论鲍曼不动杆菌多重耐药严重,临床可选择的药物极为有限,高携带ArmA甲基化酶是鲍曼不动杆菌耐药和传播流行的主要原因。Objective This paper is to detect drug-resistance and AnnA methylase of acinetobactor in order to provide evidence for treatment and prevention of nosocomial infection. Methods Aminoglycoside-resistant Acinetobactor was screened from 97 clinical isolates of acinetobactor using VITEK 2Compact. ArmA methylase gene was ampified using PCR method. The PCR products was sequenced. Results The resistant rate of more than 3 antibiotics such as cephalosporin, aminoglycoside and quinolone in Acinetobactor is about 50.3%. The detection rate of ArmA methylase in aminoglycoside-resistant Acinectobactor is about 89.6%（43/48）. ArmA methylase was not detected in five isolates with resistant phenotype. Conclusion Multidrug-resistance of Acinectobactor is severe and antibiotics for clinical selection is limited. High prevalence of ArmA methylase is main reason of drug-resistance production and clinical spread of Acinetobactor.

关 键 词：鲍曼不动杆菌 氨基糖苷类 耐药性 16SRRNA甲基化酶 

分 类 号：R378[医药卫生—病原生物学]