佛山地区产青霉素酶淋球菌和高度耐四环素淋球菌质粒谱型的研究  

The research on plasmid profi les of TEM-1 encoding gene in penicillinaseproducing Neisseria gonorrhoeae and tetM gene in high level tetracycline-resistant Neisseria gonorrhoeae in Foshan

作　　者：李燕娃 石正琪 赵培来 吴兴中[2] 潘洪亮 

机构地区：[1]佛山市皮肤病防治所,佛山528000 [2]广东省皮肤性病防治中心,广州510091

出　　处：《中国抗生素杂志》2014年第3期236-239,共4页Chinese Journal of Antibiotics

基　　金：佛山市科技发展专项资金项目(201008011)

摘　　要：目的了解佛山地区产青霉素酶淋球菌(PPNG)TEM-1基因和高度耐四环素淋球菌(TRNG)tetM基因分子流行情况。方法采用纸片酸度法检测PPNG,琼脂稀释法检测TRNG。建立PCR方法分别扩增TEM-1基因和tetM基因,电泳分析其质粒谱型。结果 98株临床分离淋球菌青霉素和四环素耐药率分别为96.9%和94.9%,共检出PPNG 34株(34.7%),TRNG 63株(64.3%)。质粒分型结果显示PPNG中亚洲型30株(88.2%),非洲型4株(11.8%),未检出多伦多/里约型,TRNG均携带荷兰型质粒。讨论佛山地区PPNG大多数携带亚洲型质粒,少见非洲型质粒,TRNG均为携带荷兰型tetM基因的流行株。Objective To investigate the molecular epidemiology of TEM-l encoding gene in penicillinase producing Neisseria gonorrhoeae and lelM gene in high level tetracycline resistant Neisseria gonorrhoeae, and assess the epidemic situation of Neisseria gonorrhoeae in Foshan. Methods PPNG were detected by paper acidometric method and TRNG by an agar dilution method. Two polymerase chain reactions were used to amplify the TEM-1 gene and tetM gene, respectively. Then analysis of the plasmid profiles of TEM-1 in PPNG and telM in TRNG were used by electrophoresis. Results The drug resistance rate ofpenicilline and tetracycline is 96.9% and 94.9%, respectively. 34 （34.7%） PPNG strains and 63 （64.3%） TRNG strains were detected in 98 clinical isolated Neisseria gonorrhoeae strains. And the results of distributing of plasmid profiles showed that 30 strains with Asian, 4 strains with African and no strain with Rio/Toronto plasmid in PPNG. Only Dutch plasmid was detected in TRNG. Conclusions Asian plasmid is the major popular in PPNG but African plasmid is rare, and all of the TRNG carrying Dutch plasmid.

关 键 词：淋球菌 青霉素酶 四环素耐药 质粒谱 

分 类 号：R378.1[医药卫生—病原生物学]