鸡肾型传染性支气管炎病毒QD株S1基因的克隆与序列分析  

Cloning and Sequence Analysis of S1 Gene of Avian Nephropathogenic Infectious Bronchitis Virus QD Isolate

作　　者：孙美玉 陈冰 邢婧珉 王寿山 吕茂杰 何召庆 杨保收 

机构地区：[1]天津瑞普生物技术股份有限公司、瑞普生物研究院生物制品研究中心,天津300308

出　　处：《中国畜牧兽医》2014年第3期87-90,共4页China Animal Husbandry & Veterinary Medicine

基　　金：瑞普研究院动物疫病流行病学调查专项基金

摘　　要：从某发病鸡场分离获得1株嗜肾型鸡传染性支气管炎病毒（infectiousbronchitisvirus，IBV），命名为QD株，据GenBank发表的IBVS1基因序列设计1对特异性引物对QD分离株s1基因进行扩增、克隆及序列测定，并将其与国内流行株及参考毒株进行比对。结果显示，IBVQD株S1基因全长为1620bp，S蛋白裂解识别位点为RRFRR。同源性分析结果表明，QD株与国内外疫苗毒株核苷酸同源性为78．8％～82．2％。遗传进化分析结果表明，QD株与国内外主要疫苗株亲缘关系较远，与近几年国内分离的肾型毒株亲缘关系最近。本试验结果表明IBV肾型毒株在中国广为流行，该毒株可作为肾型传染性支气管炎疫苗株的候选毒株。One nephropathogenic infectious bronchitis virus （IBV） strain was isolated from Qingdao city, named as QD iso late. S1 gene of the strain was amplified, cloned and sequenced. The S1 gene of QD isolate was composed of 1620 nucleotides, and a spike glycoprotein cleavage recognition site was Arg Arg-Phe-Arg Arg. The nucleotide acid similarities among the nine IBV vaccine strains and the QD strain were 78.8% to 82.2%. Phylogenetic analysis based on the S1 genes showed that QD strain and the vaccine strains belonged to different clusters, and showed larger evolutionary distances, but showed the smaller evolutionary distances with the field nephropathogenic IBV strains in China. The result showed that nephropathogenic IBV strains were widely popular in China, and the QD strain could be used as the infectious bronchitis vaccine candidate strain.

关 键 词：传染性支气管炎病毒 S1基因 序列分析 

分 类 号：Q78[生物学—分子生物学]