藏獒皮肤成纤维细胞培养与生物学特性观察  

Culture and Biological Characteristics Observation of Skin Fibroblast Cells from Tibetan Mastiff

作　　者：石凡[1,2] 杨永梅[3] 于鸿浩[2,4] 郭志林[1,2] 郭松长[2] 赵新全[2] 

机构地区：[1]青海民族大学化学与生命科学学院,青海西宁810007 [2]中国科学院西北高原生物研究所、高原生物适应与进化重点实验室,青海西宁810008 [3]青海民族大学公共管理学院,青海西宁810007 [4]榆林学院生命科学研究中心,陕西榆林719000

出　　处：《中国畜牧兽医》2014年第3期156-161,共6页China Animal Husbandry & Veterinary Medicine

基　　金：中国科学院2012年“西部之光”人才培养计划项目“藏獒繁殖生物技术研究”(科发人教字[2012]179号);财政部战略生物资源科技支撑专项“青藏高原羊模式动物及基地建设”(KSCXZ-YW-Z-0951)

摘　　要：为了研究藏獒体细胞生物学特性和保存藏獒遗传资源,采用组织块法建立了2株藏獒成纤维细胞系,对体外培养的细胞形态、增殖能力和部分生物学特性进行了分析。结果表明,体外培养的藏獒细胞呈现典型的成纤维细胞形态,生长良好,增殖能力强,测定的细胞群体倍增时间为46.1h;经免疫荧光染色,细胞表达波形蛋白;F5代细胞正常二倍体核型率为91.4%,藏獒核型2n=78,X染色体为亚中着丝粒染色体,Y染色体为端着丝粒染色体。本研究所建立的藏獒体细胞系为开展藏獒体细胞核移植研究提供了材料。n order to study biological characteristics of Tibetan Mastiff somatic ceils and preserve genetic resource, two fi- broblast cell strains from Tibetan Mastiff were established by tissue culture. In vitro culture cell morphology, hyperplasia abil- ity and some biological characteristics were researched. Tibetan Mastiff ceils in vitro culture appeared typical morphology of fi- broblast cells, grew well and had strong hyperplasia ability. Population doubling time of the cells was 46.1 h. Vimentin ex- pression in the cells was confirmed by immunofluorescence staining. The rate of F5 cells with normal diploidy （2n= 78） was 91.4%. Moreover, longer X chromosome belonged to submetacentric chromosome, but Y chromosome did telocentric chromo- some. Tibetan Mastiff cell strains eslablished in the study offered the materials for the Tibetan Mastiff somatic cell nuclear transfer research.

关 键 词：藏獒 细胞培养 成纤维细胞 免疫荧光染色 

分 类 号：S823.3[农业科学—畜牧学]