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机构地区:[1]盐城市农产品质量监督检验测试中心,江苏盐城224002 [2]扬州大学动物科学与技术学院,江苏扬州225009
出 处:《中国畜牧兽医》2014年第3期170-173,共4页China Animal Husbandry & Veterinary Medicine
摘 要:本试验研究了不同浓度的大豆苷(daidzin)和染料木苷(genistin)对山羊乳腺上皮细胞(mammary gland epithelial cells,MEC)增殖的影响,主要采用MTT法研究不同浓度的大豆苷和染料木苷刺激影响细胞增殖的状况。结果表明,2种苷纳克级别浓度组较微克级别浓度组对细胞增殖的影响更明显;处理细胞48h较24和72h对细胞增殖的影响更明显;10ng/mL大豆苷和10、100ng/mL染料木苷均能显著促进山羊MEC增殖(P<0.05)。The experiment was aimed to study the effects of different concentrations of daidzin and genistin on proliferation of goat mammary gland epithelial ceils, the test was adopted the method of MTT. The results revealed that the ng level of con- centration had more effects on cells than theμg level of concentration. And when cells were treated with the two materials for 48 h, the effects were greater than for 24 and 72 h. The highest poteney was detected in daidzin (10 ng/mL) and genistin (10 and 100 ng/mL) compared with the control group.
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