吐鲁番斗鸡攻击行为谱的编制与单胺氧化酶A基因的SNP分析  

Establishment of Turpan Gamecock Aggression Behavior Spectrum and SNP Analysis of Monoamine Oxidase AGene

作　　者：李一丹[1] 刘秀霞[1] 李应生[1] 王磊[1] 贾薇[1] 李兴明[1] 钟新献[1] 李岩[1] 廖和荣[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《中国畜牧兽医》2014年第3期182-187,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31360545);石河子大学高层次人才项目(RCZX201101)

摘　　要：为探讨评定吐鲁番斗鸡攻击行为表型强弱的行为谱和影响吐鲁番斗鸡攻击行为相关基因的SNP,采用镜像自斗法和配对打斗法分别观测相同的12只吐鲁番成年斗公鸡的攻击行为表型,并对103只吐鲁番斗鸡和71只新罗曼鸡单胺氧化酶A(monoamine oxidase A,MAOA)基因的第1、2外显子进行了PCR-SSCP分析。结果显示,2种打斗法的攻击行为表型的强弱排序基本一致,表明所编制的攻击行为的行为谱可用于吐鲁番斗鸡攻击行为强弱的表型评定;MAOA1在吐鲁番斗鸡和新罗曼鸡上检测到的多态性一致,而在MAOA2上检测到多态性并不完全一致,推测MAOA2位点与吐鲁番斗鸡的攻击行为具有一定的相关性。To investigate and assess the aggressive behavior phenotypic spectrum of Turpan gamecock and SNP of their be havior related genes, this study adopted the methods including mirror fighting and pair fighting to observe aggressive behavior phenotypes of 12 adult Turpan gamecock, and PCR SSCP analysis about monoamine oxidase A （MAOA） gene in exons 1 and 2 of 103 Turpan gamecock and 71 New Romain chicken. The results showed that both methods of fighting strength of aggressive behavior phenotype sort basically the same, indicating that the behavior of aggressive behavior spectrum could be used for the preparation of aggressive behavior in Turpan gamecock fighting strength of phenotypic assessment. MAOA1 polymorphism in Turpan gamecock and New Romain was no difference after detection, but on the MAOA2, the polymorphism was not entirely consistent, suggesting MAOA2 might be had a certain correlation with Turpan gamecock aggressive behavior.

关 键 词：吐鲁番斗鸡 攻击行为 行为谱 单胺氧化酶A PCR—SSCP 

分 类 号：S813.3[农业科学—畜牧学]