荧光光谱法测定癌光啉在BN大鼠体内的药代动力学  

Study on pharmacokinetics of PSD-007 in BN rats by fluorescence detection methods

作　　者：阴慧娟[1] 陈洪丽[1] 顾立超 李迎新[1] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192 [2]河北联合大学附属医院血液内科,唐山063000

出　　处：《国际生物医学工程杂志》2014年第1期18-21,共4页International Journal of Biomedical Engineering

基　　金：国家自然科学基金青年项目（81201819）

摘　　要：目的 研究荧光光谱法检测癌光啉在BN大鼠体内的药代动力学.方法 BN大鼠尾静脉注射5 mg/kg体质量的癌光啉,于给药后的10 min、30 min、1h、2h、4h、8h、24 h、48 h和72 h眼眶后静脉丛取血,以荧光光谱仪测定大鼠血浆中癌光啉浓度,用DNS2.0处理血药浓度-时间数据,分析房室模型,得到最佳房室模型和药代动力学参数.结果 BN大鼠静脉注射的时量曲线符合三室模型.主要药代动力学参数：t1/2a=0.096 h,t1/2β=1.299 h,t1/2=19.387 h,V1=0.259 L/kg,A UC=15.263 mg/（L·h）.结论 荧光光谱法检测光敏剂血药浓度操作简单,耗时短,灵敏度和特异性高.癌光啉在BN大鼠体内吸收快,起效快,无蓄积现象.Objective To investigate the pharmacokinetic characteristics of PSD-007 in BN rats.Methods Blood of the BN rats was collected from the inner canthus after iv administration of PSD-007,and the plasma drug concentrations at different times were determined by fluorescent spectrometry.The best compartment model and the pharmacolinetic were calculated by the software of DNS 2.0.Results The elimination process of PSD-007 fitted three-compartment open model after iv administration.The principal pharmacokinetic parameters were t1/2α=0.096 h,t1/2β=1.299 h,t12γ=19.387 h,V1=0.259 L/kg,A UC=15.263 mg/（L ·h）.Conclusions The sensitivity of fluorescent spectrometry was high and the operation is sinple and the progress is short.PSD-007 has fast absorption,quick effect and elimination without accumulation phenomenon.

关 键 词：癌光啉 BN大鼠 荧光光谱 药代动力学 

分 类 号：O657.3[理学—分析化学] R969.1[理学—化学]