外源性LIF和IL-1对围着床期小鼠子宫内膜整合素β_3亚基表达的调节  

Regulation of external LIF on the expression of integrin β_3 in the mouse endometrium during peri-implantation period

作　　者：张炜[1] 周剑萍[1] 刘银坤[2] 朱瑾[1] 

机构地区：[1]上海医科大学妇产科医院,上海200011 [2]上海医科大学中山医院肝癌研究所,上海200000

出　　处：《中国免疫学杂志》2001年第2期93-95,共3页Chinese Journal of Immunology

基　　金：上海市卫生局科技基金!资助 (96 42 9)

摘　　要：Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin 1(IL 1) on the expression of integrin β 3 in the mouse endometrium during peri implantation period and explore the mechanism which LIF participate in embryonic implantation.Methods:The mice were injected peritoneally with LIF,IL 1 or IL 1ra at different administration on day 2～4 of pregnancy.The level of integrin β 3 protein was measured with Westernblot method and mRNA measured with RT PCR.Results:The levels of integrin β 3 protein and mRNA were increased by LIF and IL 1(P<0.05) and which relied on administration.The level of integrin β 3 mRNA was decreased by IL 1ra (P<0.05).Conclusion:LIF and IL 1 of cytokines can improve the expression of integrin β 3 in the peri implantation endometrium of mouse.Both of them may take part in implantation by regulating cell adhesion molecules.Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin 1(IL 1) on the expression of integrin β 3 in the mouse endometrium during peri implantation period and explore the mechanism which LIF participate in embryonic implantation.Methods:The mice were injected peritoneally with LIF,IL 1 or IL 1ra at different administration on day 2～4 of pregnancy.The level of integrin β 3 protein was measured with Westernblot method and mRNA measured with RT PCR.Results:The levels of integrin β 3 protein and mRNA were increased by LIF and IL 1(P<0.05) and which relied on administration.The level of integrin β 3 mRNA was decreased by IL 1ra (P<0.05).Conclusion:LIF and IL 1 of cytokines can improve the expression of integrin β 3 in the peri implantation endometrium of mouse.Both of them may take part in implantation by regulating cell adhesion molecules.

关 键 词：白血病抑制因子 整合素Β3亚基 子宫内膜 着床 白细胞介素-1 围着床期 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学] R392.9[医药卫生—基础医学]