黄芩素对副溶血弧菌噬菌体裂解宿主的影响研究  

Study on the lysis effects on Vibrio parahaemolyticus phage and its host by baicalein

作　　者：贺美[1,2] 柏仕杰[2,3] 邱德全[2] 

机构地区：[1]长江大学地球环境与水资源学院,武汉430100 [2]广东海洋大学水产学院,湛江524025 [3]中国科学院水生生物研究所,武汉430072

出　　处：《生态科学》2013年第5期576-581,共6页Ecological Science

基　　金：国家自然科学基金资助项目(21207020);广东省海洋渔业局重点课题(A2010001-015(a);A201008E03;A201208E01);广东省科技厅课题能(2009B020309002;2011B020307011);广东省科技计划项目(2012B020415006);长江大学科研发展基金

摘　　要：论文以副溶血弧菌噬菌体为研究对象,通过研究黄芩素对副溶血弧菌噬菌体裂解宿主的影响来反映黄芩素的抗病毒效果,同时还研究了将黄芩素制备成环糊精包合物后对副溶血弧菌噬菌体裂解其宿主作用的影响。结果表明,黄芩素及其环糊精包合物均抑制了副溶血弧菌噬菌体对其宿主的裂解,且抑制作用均与它们的浓度与作用时间(一定时间范围内)成正相关;将黄芩素制备成环糊精包合物后有利于黄芩素对副溶血弧菌噬菌体裂解抑制作用的增强,在相同浓度下,黄芩素环糊精包合物对副溶血弧菌噬菌体作用60 min就能完全抑制了副溶血弧菌噬菌体对其宿主的裂解作用,而黄芩素则需要120 min。可见,利用副溶血弧菌噬菌体对其宿主的裂解作用来研究一些药效成分的抗病毒作用是一种简单、方便、成本较低的有效方法。Vibrio parahaemolyticus phage （a representative and simple virus） was used to study the antiviral effects of baicalein by investigating the impact on the lysis effects of V. parahaemolyticus phage and its host. Then the influence of baicalein with Hydroxypropyl-13- Cyclodextrin on the lysis effects of V. parahaemolyticus phage and its host was also studied because of the low solubility and the instability of baicalein. The results showed that both baicalein and its cyclodextrin complexes inhibited the lysis of V.parahaemolyticus phage on it host and both the inhibition effects were positively correlated with their concentrations and effect time. It was beneficial for Baicalein to play its inhibition effects on the lysis of V. parahaemolyticus phage and its host after Baicalein was prepared as its cyclodextrin complexes. The lysis of V. parahaemolyticus phage to its hosts was totally inhibited after cyclodextrin complexes of Baicalein was added for 60 minutes, however that of baicalein was for 120 min. Thus it can be seen that it is a simple, convenient, low-cost and effective method to use the lysis effects of the If. parahaemolyticus phage on its host to study the antiviral effect of some active ingredients.

关 键 词：副溶血弧菌 副溶血弧菌噬菌体 黄芩素 抗病毒作用 环糊精 

分 类 号：Q93-3[生物学—微生物学]