皂帽花果实挥发油成分及抗肿瘤活性研究  

Composition and Antitumor Activities of Essential Oil from the Fruits of Dasymaschalon trichophorum

作　　者：李小宝[1,2] 陈光英[1,2] 宋小平[1,2] 韩长日[1,2] 郑彩娟[1,2] 

机构地区：[1]海南师范大学省部共建-热带药用植物化学教育部重点实验室 [2]海南师范大学化学与化工学院,海南海口571158

出　　处：《中药材》2013年第11期1786-1788,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81160391,21166009);973计划前期专项(2011CB512010)

摘　　要：目的:分析研究皂帽花果实挥发油的主要成分及其抗肿瘤活性。方法:采用水蒸气蒸馏法从皂帽花果实中提取挥发油,利用气相色谱-质谱对其化学成分进行分析。MTT(四甲基偶氮唑盐)法检测皂帽花提取物对细胞SPCA-1、BEL-7402、SGC-7901和K-562增殖抑制作用。结果:分离出55个峰,鉴定出30种化学成分,占挥发油总量的74.98%。其中主要成分为橙花叔醇(19.887%)、石竹烯(16.714%)和β-月桂烯(9.29%)。抗肿瘤活性结果表明皂帽花果实挥发油对4种肿瘤细胞株均显示一定的抑制活性,其中对肺腺癌细胞SPCA-1的抑制活性最强,其IC50值为6.61μg/mL。结论:该实验为皂帽花的进一步开发研究提供了科学依据。Objective：To study the chemical components and antitumor activities of essential oil from the fruits of Dasymaschalon trichophorum. Methods ： The essential oil from the fruits of Dasymaschalon trichophorum was extracted by steam-stilling and analyzed by GC-MS. The inhibitory effect of extract ofDasymaschalon trichophorum on SPCA-1 ,BEL-7402,SGC-7901 and K-562 cell proliferation was assayed using the MTF. Results ： GC-MS result showed that there were 55 peaks and 30 compounds were identified which weighed 74. 98%. The major components were nerolidol（ 19. 887% ） ,caryophyllene（ 16. 714% ）and ]3-myrcene（9. 29% ）. The essential oil exhibited strong antitumor activity against SPCA-1 human tumor cell line with IC50 value of 6. 61 μg/mL. Conclusion： This experiment provides scientific foundation for further utilization of Dasymaschalon trichophorum.

关 键 词：皂帽花 挥发油 气相色谱-质谱技术 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]