河南齿叶橐吾根化学成分研究  

Study on the Chemical Constituents from the Roots of Ligularia dentata in Henan

作　　者：李爽[1] 杨茹[2] 曾献磊[2] 王彩芳[2,3] 

机构地区：[1]郑州人民医院药学部,河南郑州450053 [2]郑州大学药学院,河南郑州450008 [3]北京师范大学化学学院,北京100875

出　　处：《中药材》2013年第11期1789-1791,共3页Journal of Chinese Medicinal Materials

基　　金：公益性行业(农业)科研专项(201103027);"重大新药创制"科技重大专项(2011ZX09307-002-01);河南省教育厅自然基金(2011A350003)

摘　　要：目的：研究河南齿叶橐吾根的化学成分。方法：用硅胶柱色谱、SephadexLH-20凝胶柱色谱结合溶剂结晶法分离纯化河南齿叶橐吾根的化学成分，通过NMR及MS波谱法确定化合物结构，并采用RP—HPLC法分析各化合物在该植物根部乙醇提取物中的存：i芏情况。结果：从河南齿叶橐吾根部乙醇提取物中分离并鉴定出4个艾里莫芬烷型倍半萜化合物，分别为：3-羰基-8“-羟基-10all-艾里莫芬烷-1，7-二烯-8，1帮-内酯（1）、3-羰基-艾里莫芬烷-1，7-二烯-8，1举-内酯（2）、3-羰基-8“-乙氧基-10all-艾里莫芬烷-1，7-二烯-8，12-内酯（3）及呋喃橐吾酮（4）。结论：化合物1～4为首次从该植物中分离得到。HPLC法分析结果表明，除化合物3外的其余化合物在该植物根部含量较大，化合物3从新制备的乙醇提取液中几乎检测不到，推测可能是在提取过程中由其他化合物转化而来。Objective：To study the chemical constituents from the roots of Ligularia dentata in Henna. Methods：Chemical constituents from the roots of Ligularia dentata in Henan were isolated and purified by silica gel, Sephadex LH-20 column chromatography and solvent crystallization and their structures were identified by spectral methods. Results： Four eremophilane-type sesquiterpenoids were obtained and determined as 3-oxo-8①-hydroxy-10αH-eremophilan-1,7-dien-8,12β-olide （ 1 ） , 3-oxo-eremophil-1,7-dien-8, 12β-olide （2） ,3-oxo-8α-ethoxy-10αH-eremophil-1,7-dien-8,12-olide （ 3 ） and furanoligularenone （ 4 ）. Conclusion ： Compound 1 - 4 are isolated from this plant for the first time and their contents in the roots are high by HPLC analysis except for compound 3. Compound 3 is hardly detected in the fresh EtOH extract and might be derived from other compounds during the extraction.

关 键 词：齿叶橐吾根 倍半萜 分离鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]