黑顺片总生物碱对番泻叶致小鼠腹泻及大鼠离体小肠平滑肌运动的影响  

Effects of Heishunpian Total Alkaloids on Cassia acutifolia Induced Mice Diarrhea and Contraction of Isolated Intestinal Smooth Muscle in Rats

作　　者：邵峰[1] 陈慧娟[1] 刘荣华[1] 侯永春[1] 任刚[1] 黄慧莲[1] 唐芳瑞[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中药材》2013年第11期1805-1809,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30801520);江西省教育厅项目(GJJ10212);江西省卫生厅项目(2010A29;2012A153)

摘　　要：目的:考察黑顺片总生物碱对番泻叶致小鼠腹泻及大鼠离体小肠平滑肌运动的影响。方法:采用番泻叶致小鼠腹泻模型,以小肠推进率为考察指标,观察黑顺片总生物碱对小鼠腹泻的影响;采用大鼠离体小肠平滑肌实验,以小肠平滑肌收缩的振幅和张力为考察指标,观察黑顺片总生物碱对小肠平滑肌运动的影响及其作用机理;采用HPLC法,分析黑顺片总生物碱中的化学成分。结果:黑顺片总生物碱能显著减慢腹泻小鼠小肠推进运动(P<0.05),并能剂量依赖性降低正常大鼠离体小肠平滑肌收缩的振幅和张力,对氯化乙酰胆碱、甲硫酸新斯的明所兴奋的小肠平滑肌运动均具有一定的抑制作用,但对硫酸阿托品所抑制的小肠平滑肌运动无明显影响。经HPLC分析,苯甲酰新乌头碱、苯甲酰乌头碱及苯甲酰次乌头碱均是黑顺片总生物碱的主要化学成分。结论:黑顺片总生物碱对腹泻小鼠具有一定的止泻作用,其作用机理可能与抑制M胆碱受体有关。Objective ： To observe the effects of Heishunpian total alkaloids on Cassia acutifolia induced mice diarrhea and contrac- tion of isolated intestinal smooth muscle in rats. Methods：The experiment was carried out with Cassia acuti）blia induced mice diarrhea model, small intestinal propulsive rate in mice was used to valuate the effect of Heishunpian total alkaloids on diarrhea mice ;The effects of Heishunpian total alkaloids on contraction of isolated intestinal smooth muscle in rats and its mechanism were investigated by monito- ring amplitude and tension of isolated intestinal smooth muscle in rats ;The chemical constituents of Heishunpian total alkaloids were an- alyzed by HPLC. Results ： Heishunpian total alkaloids could significantly slow down intestine propulsive motility in diarrhea mice （ P 〈 0. 05）, and reduce amplitude and tension of isolated small intestinal smooth muscle contraction in normal rats in dose - dependent man- ner. Heishunpian total alkaloids had a certain inhibitory effect on acetylcholine chloride and neostigmine methylsulfate strengthening small intestine smooth muscle movement, howe, vet, had no significantly effect on Atropine sulfate inhibiting small intestine smooth muscle movement. By HPLC analysis, benzoylaconitine, benzoylmesaconitine, benzoylhypaconitine were the main chemical constituents of Heishunpian total alkaloids. Conclusion：Heishunpian total alkaloids have a certain role of antidiarrheal and its mechanism may be re- lated to antagonizing muscarinic receptors.

关 键 词：黑顺片 总生物碱 小鼠小肠推进运动 大鼠离体小肠平滑肌 单酯型乌头碱 

分 类 号：R285.5[医药卫生—中药学]