野菊花总黄酮对人骨肉瘤Saos-2细胞增殖和凋亡的影响  

Effects of Total Flavones of Chrysanthemum indicum on Proliferation and Apoptosis of Human Osteosarcoma Saos-2 Cells

作　　者：魏强强[1,2] 殷嫦嫦[3] 周湖燕[2] 何丁文[1,2] 梁广胜[1,2] 刘玉亮[1,2] 殷明[1] 

机构地区：[1]南昌大学第二附属医院,江西南昌330006 [2]南昌大学,江西南昌330006 [3]九江学院,江西九江332000

出　　处：《中药材》2013年第11期1823-1827,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨野菊花总黄酮对人骨肉瘤细胞增殖和凋亡的影响及其作用机制。方法:人骨肉瘤Saos-2细胞经野菊花总黄酮在不同浓度及不同时间作用后,倒置相差显微镜下观察细胞形态及细胞密度的变化,并采用CCK法检测野菊花总黄酮对细胞增殖的影响;流式细胞仪和逆转录-聚合酶链反应(RT-PCR)检测不同药物浓度作用细胞48 h后的凋亡率以及凋亡相关基因Caspase-3、BCL-2、BAX的表达情况。结果:野菊花总黄酮对Saos-2细胞的增殖有明显抑制作用,并呈剂量和时间依赖性,显微镜下观察可见细胞形态变圆,体积变小,内部颗粒增多等凋亡的形态变化。流式细胞仪检测细胞的凋亡率与药物浓度正相关。药物作用细胞48 h后,Caspase-3和BAX表达上调,BCL-2表达下降,BCL-2/BAX比率下降。结论:野菊花总黄酮对人骨肉瘤Saos-2细胞具有增殖抑制及诱导凋亡的作用,其机制可能与下调BCL-2/BAX比率而激活Caspase-3相关。Objective：To study the effects of total flavones of Chrysanthemum indicum on proliferation and apoptosis of human os- teosarcoma Saos-2 cells and its mechanism. Methods ： The effect of the total flavones of Chrysanthemum indicum on the proliferation of human osteosarcoma Saos-2 cells was detected by CCK assay, and the morphological changes of cells treated with total flavones of Chrys- anthemum indicum were observed using contrast microscope. Flow cytomerty was performed to analyze the apoptotic rate of the cells, and the gene expression levels of Caspase-3, BCL-2, BAX were detected by RT-PCR. Results ： The total flavones of Chrysanthemum in- dicum suppressed the proliferation of osteosarcoma cells in a dose-and time-dependent manner. Under a microscope observation of cell morphology, the volume became smaller , the number of internal particles was increased. Cell apoptosis rate was positively related to the drug concentration. After treated for 48 hours, Caspase-3 and BAX expression were up-regulated, BCL-2 and BCL-2/BAX were de- creased. Conclusion ：The total flavones of Chrysanthemum indicum can inhibit the proliferation of osteosarcoma cell line Saos-2 by indu- cing cell apoptosis, the mechanism of which might be related with reducing BCL-2/BAX and activating Caspase-3.

关 键 词：野菊花总黄酮 骨肉瘤 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]