血管内皮生长因子与妊娠高血压综合征发病的相关性研究  

Study on association of vascular endothelial growth factor with the pathogenesis of pregnancy induced hypertension

作　　者：程忠平[1,2] 林其德[2] 沈仲毅 刘伟[2] 陈珠萍[2] 汪希鹏[2] 

机构地区：[1]上海市杨浦区中心医院妇产科,200001 [2]上海第二医科大学附属仁济医院妇产科 [3]上海免疫研究所

出　　处：《中华妇产科杂志》2001年第2期72-75,T001,共5页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨血管内皮生长因子 (VEGF)与妊娠高血压综合征 (妊高征 )发病的相关性。方法　采用酶联免疫吸附法检测 2 3例妊高征患者 (妊高征组 )外周血及其新生儿脐静脉血VEGF水平 ,定量免疫组化及定量逆转录聚合酶链反应检测患者胎盘和蜕膜组织中VEGF表达情况 ,并以 2 0例健康孕妇 (正常妊娠组 )为对照。结果　 (1)妊高征组外周血VEGF水平为 [(10 4± 3 8)ng/L , x±s,下同 ],明显低于正常妊娠组的 (17 0± 9 3)ng/L。 (2 )胎盘合体滋养细胞及蜕膜间质滋养细胞均有VEGF强阳性染色。经计算机扫描图像处理 ,正常妊娠组绒毛合体滋养细胞VEGF表达为 (75 0± 9 0 )平均灰度 ,间质细胞为 (6 0 5± 6 4)平均灰度 ,均显著高于妊高征组的 (6 9 0± 8 9)平均灰度和 (5 5 0±7 3)平均灰度。正常妊娠组VEGF表达为 (4 5 4± 4 0 )平均灰度 ,显著高于妊高征组的 (4 2 5± 3 8)平均灰度。 (3)胎盘、蜕膜组织均有 3种不同片断长度的VEGFmRNA扩增 ,正常妊娠组胎盘组织VEGF总峰度比为 2 8± 1 0 ,较妊高征组 (4 6± 3 2 )极明显降低 (P <0 0 1)。正常妊娠组蜕膜组织VEGF总峰度比为 3 9± 1 5 ,也较妊高征组 (6 3± 2 9)极明显降低 ,(P <0 0 1)。结论　妊高征患者VEGF表达障碍发生在蛋白质翻译和表达水平 ;Objective To investigate relationship between the vascular endothelial growth factor (VEGF) and the pathogenesis of pregnancy induced hypertension syndrome (PIHs) Methods Twenty three women with PIHs and twenty normal pregnant women were studied The levels of VEGF in maternal and umbilical venous serum, placenta and decidual tissue from normal pregnancy and PIHs women were detected by enzyme linked immunosorbent assay (ELISA) and immunohistochemisty Furthermore, the expression of VEGF mRNA in placenta and decidua was examined by reverse transcriptase polymerase chain reaction (RT PCR) Results (1) The levels of VEGF in maternal blood were significantly lower in PIHs women than that in normal pregnancies wonen (10 4±3 8) ng/L and (17 0±9 3) ng/L ( P <0 01) (2) The average gray of VEGF was determined on sections of placenta and decidua from PIHs or pregnancies women by computer image analysis The expression of VEGF in placenta and decidua (69 0±8 9) and (55 0±7 3) was decreased significantly in PIHs women than that in normal pregnancies wonen (75 0±9 0) and (60 5±6 4, P <0 05) (3) The transcription levels of the VEGF mRNA in placenta and decidua were increased significantly in PIHs women than those in normal pregnancies women (4 6±3 2 vs 2 8±1 0;6 3±2 9 vs 3 9±1 5, P <0 01) Conclusions These findings suggested that the abnormal expression of VEGF in PIHs occured within the low stream of genic transcription It was considered that the abnormal expression of VEGF may play an important role in the pathogenesis of plcental ischemia in PIHs

关 键 词：妊娠并发症 心血管 高血压 内皮生长因子 淋巴因子 

分 类 号：R714.246[医药卫生—妇产科学]