东方田鼠天然抗日本血吸虫抗体及IgG亚类的初步研究  

PRELIMINARY STUDY ON NATURAL ANTIBODIES AND IgG SUBCLASSES AGAINST SCHISTOSOMA JAPONICUM IN MICROTUS FORTIS

作　　者：蒋守富[1] 魏梅雄[1] 林矫矫[2] 潘彩娥[1] 李浩[2] 曹琳[1] 何艳燕[1] 傅志强[2] 邱倩雯[1] 王梅芳[1] 

机构地区：[1]上海市疾病预防控制中心,上海200031 [2]中国农业科学院上海家畜寄生虫病研究所

出　　处：《中国血吸虫病防治杂志》2001年第1期1-3,共3页Chinese Journal of Schistosomiasis Control

基　　金：国家科技部"九五"攻关项目! (编号 :96 -A2 3-0 6 -0 4);上海市科技发展基金项目! (编号 :9844190 74)资助

摘　　要：目的　研究东方田鼠天然抗日本血吸虫抗体及其 Ig G亚类。方法　采用 EL ISA试验 ,分别检测室内繁殖的东方田鼠、Balb/ c和昆明种小鼠血清中 8种抗童虫 (SSA)、抗成虫 (SAWA)和抗虫卵 (SEA)抗体及 Ig G亚类。结果　首次发现东方田鼠抗 - SSA Ig G3水平明显高于 Balb/ c和昆明种小鼠 ,分别为后者的 1.44倍和 1.5 5倍 ,而抗 - SAWA Ig G3和抗 - SEA Ig G3水平则无明显增高。结论　东方田鼠体内确实存在天然抗日本血吸虫抗体 。Objective ”BZ]To study the serum antibodies and IgG subclasses against Schistosoma japonicum ”BZ]in Microtus fortis (Mf). Methods To study the serum antibodies and IgG subclasses against Schistosoma japonicum ”BZ]in Microtus fortis (Mf). Methods in Microtus fortis (Mf). Methods [WT5”BZ]Different isotypes of antibodies and IgG subclasses against soluble schistosomulum antigen (SSA), soluble adult worm antigen (SAWA) and soluble egg antigen (SEA) in laboratory bred Microtus fortis, ”BZ]Balb/c and Kunming mice (Km) were detected by indirect enzyme linked immunosorbent assay (ELISA).Results ”BZ] The level of anti SSA IgG 3 subclass antibody was significantly higher in Mf Balb/c and Kunming mice (Km) were detected by indirect enzyme linked immunosorbent assay (ELISA).Results The level of anti SSA IgG 3 subclass antibody was significantly higher in Mf The level of anti SSA IgG 3 subclass antibody was significantly higher in Mf than that in Balb/c or Km. The mean optical density (OD) value of anti SSA IgG 3 subclass in Mf

关 键 词：东方田鼠 日本血吸虫 抗体 IGG亚类 

分 类 号：R531.21[医药卫生—内科学] R383.24[医药卫生—临床医学]