山西白猪SLA-DQB基因多态性与生产性状的关联分析  

Polymorphism of SLA-DQB gene and its relationship for growth traits in Shanxi white pigs

作　　者：杨文平[1] 张家琦[1] 曹果清[2] 周忠孝[2] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063000 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《河南农业大学学报》2014年第1期58-60,65,共4页Journal of Henan Agricultural University

基　　金：山西省科技攻关项目(991016;20080311031);科技部农业科技成果转化资金项目(2008GB2A300032)

摘　　要：采用PCR-RFLP法对山西白猪SLA-DQB基因外显子2多态性和生产性状的关联分析进行了研究.结果表明,山西白猪SLA-DQB基因外显子2经HaeⅢ酶切后产生5种等位基因(A,B,C,D和E),6种基因型(AA,BB,CC,EE,AC和CD);最小二乘分析结果表明,EE基因型与其它2种基因型比较有较大的初生重,同AA和CC型比较差异显著(P<0.05),3种基因型在初生重的大小排列顺序为CC<AA<EE;28日龄体重、6月龄重和6月龄体尺等基因型间差异不显著(P>0.05).从生产性状看,本研究中EE基因型是优势基因型.因此,推测SLADQB基因对个体的初生重存在一定的影响.Polymorphism of the exon 2 of SLA （ swine leukocyte antigen）-DQB gene in Shanxi white pigs was analyzed by PCR-RFLP method. The results showed that the exon 2 of SLA-DQB gene digested by Hae HI could be divided into five kinds of alleles （A, B, C,D and E） and six kinds of genotypes （AA,BB,CC,EE,AC and CD）. Using least square analysis , individuals of the EE genotype had significantly higher （P 〈 0.05 ） birth weight than those of AA and CC genotypes , with the order being CC 〈 AA 〈 EE. No significant difference existed in 28-day body weight, 6-month body weight and 6-month body size （P 〉 0.05）. As the growth traits of Shanxi white pigs, genotype EE was the superior one. These results suggest that SLA-DQB gene has significant effects on individual birth weight.

关 键 词：山西白猪 SLA—DQB基因 PCR—RFLP 生产性状 

分 类 号：S828[农业科学—畜牧学]