减毒水痘病毒对乙型肝炎病毒复制的影响  

The effect of attenuated varicella-zoster virus on replication of HBV

作　　者：杨湛[1] 邓学龙[2] 张奉学[2] 吴婉芬[1] 雷春亮[1] 朱宇同[2] 

机构地区：[1]广州市第八人民医院肝病研究所,510060 [2]广州中医药大学中药抗病毒研究室

出　　处：《中华肝脏病杂志》2001年第1期28-30,共3页Chinese Journal of Hepatology

基　　金：广东省科委重点科技攻关项目!(960038)

摘　　要：目的　观察减毒水痘病毒对乙型肝炎病毒复制的影响。方法　分别用减毒水痘病毒接种鸭乙型肝炎模型和ＨｅＰＧ２ ２．２．１５细胞，以斑点杂交和ＥＩＡ方法分别检测鸭血清中ＤＨＢＶ ＤＮＡ和细胞培养上清液中ＨＢｓＡｇ、ＨＢｅＡｇ的含量。结果　减毒水症病毒两个剂量组均显示鸭血清病毒量下降，２００ｐｆｕ／ｋｇ组在给药后第１０天和停药５ｄ时， ＤＨＢＶ ＤＮＡ吸光度（Ａ）平均值分别由给药前１．１７ ± ０．２９降至０．５９ ± ０．４５和　０．２１± ０．２１，相比差异有显著意义（ｔ＝３．５１， Ｐ＜０．０１和ｔ＝ ７．５４，Ｐ＜０．００１）；４００ｐｆｕ／ｋｇ组在给药后第５、１０天ＤＨＢＶ ＤＮＡ无下降，停药５ｄ时ＤＨＢＶ ＤＮＡ Ａ平均值由给药前０．７０± ０．２５降至０．３２ ± ０．１７，差异有显著意义（ｔ＝３．５８，Ｐ＜０．０１）；减毒水症病毒对２．２．１５细胞分泌ＨＢｅＡｇ、ＨＢｓＡｇ均有抑制作用，最大抑制率分别为６１％和３３％，对ＨＢｅＡｇ的抑制较ＨＢｓＡｇ强。结论　减毒水症病毒在鸭乙型肝炎模型上可以显著降低血清ＤＨＢＶ ＤＮＡ水平；在体外能够直接抑制２．２．１５细胞分泌ＨＢｓＡｇ、ＨＢｅＡｇ，提示该病毒可能对乙型肝炎病毒复制有?Objective To observe the effect of attenuated varicella-zoster virus (VZV) on replication of HBV in vitro and vivo. Methods The attenuated VZV were inoculated into the ducks infected with DHBV and into the cultural HepG2 2.2. 15 cells, respectively. DHBV DNA, HBsAg and HBeAg were detected from the duck serum and cell cultural fluid by dot-blot hybridization and EIA. Results The decline of DHBV DNA in duck serum was found in two dosage groups. Compared with pre-treatment, the level of AD value in 200 pfu/kg group was significantly decreased from 1. 17 ± 0. 29 to 0.59 ± 0.45(10 d after VZV treatment) and 0.21 ± 0.21(5 d after withdrawal of VZV) (t=3.51, p<0.01 and t=7.54, P < 0.001). The level in 400 pfu/kg group was from 0.70 ± 0.25 to 0.32 ± 0. 17 on the 5th day of withdrawal of VZV(t=3.58, P<0.01), respectively after treatment. The inhibitory rates of HBeAg and HBsAg were 61% and 33%, respectively. The effect on HBeAg seems to be more obvious than on HBsAg. Conclusion Attenuated VZV could significantly decrease serum DHBV DNA in DHBV-infected ducks and directly inhibit the HBeAg and HBsAg secreted by 2.2. 15 cells, suggesting that VZV seems to interfere or inhibit the replication of DHBV.

关 键 词：减毒水痘病毒 乙型肝炎病毒 鸭乙型肝炎病毒 病毒复制 乙型肝炎 

分 类 号：R373[医药卫生—病原生物学]