转铁蛋白偶联荧光纳米钻石靶向体系的制备及HeLa细胞吸收机制研究  

Preparation of Transferrin-Coupled Fluorescence Nanodiamond and an Investigation of Its Uptake Mechanism by HeLa Cells

作　　者：田志梅[1] 曹瑞霞[2] 王东新[1] 李林[1] 王青[2] 王志琴[2] 李英奇[1,2] 

机构地区：[1]山西大学分子科学研究所、化学生物学与分子工程教育部重点实验室,太原030006 [2]山西大学化学化工学院,太原030006

出　　处：《太原理工大学学报》2014年第2期226-232,共7页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金资助项目(21071091);山西省科技攻关计划(社会发展)资助项目(20130313021-1);山西省自然科学基金资助项目(2009011012-3);山西省归国留学基金资助项目(201011)

摘　　要：以荧光纳米钻石(FND)作为药物载体和探针,转铁蛋白(Tf)作为靶向配体,聚赖氨酸(PL)做桥梁,制备了荧光纳米钻石-聚赖氨酸-转铁蛋白(FND-PL-Tf)靶向纳米颗粒;以人宫颈癌细胞(HeLa cells)为体外模型,研究靶向纳米颗粒与细胞的作用,探讨细胞摄取纳米颗粒的转运机制,为纳米颗粒靶向输送药物和肿瘤检测提供有价值的理论依据。结果表明细胞摄取FND-PL-Tf纳米颗粒的数量与颗粒浓度、时间及纳米颗粒表面偶联转铁蛋白量有关;由物理吸附或共价偶联Tf获得的FND-PL-Tf纳米颗粒均为网格蛋白决定、转铁蛋白受体介导机制进入细胞。这些研究表明FND-PL-Tf纳米颗粒具有潜在的靶向输送药物和肿瘤靶向检测功能。The biological applications of fluorescent nanodiamonds （FND） have aroused wide concerns. In this work, the targeting system FND-L-f was prepared with the fluorescent nanodiamond （FND） as a platform for delivery of drug and a probe, transferrin （Tf） as a targeted ligand and polylysine （PL） as a bridge. The interaction between the FND-L-f nanoparticles and human cervical carcinoma cells （HeLa cells） as an in vitro model was studied to explore the cellular uptake mechanism of nanopartieles, which could provide valuable theoretical basis for the targeted drug delivery and cancer detection. The results display that the cellular uptake was dependent on FND-L- Tf particle concentration, transferrin ligand density, and dosing time. In addition, the FND-PL-Tf nanoparticles prepared from FND-PL nanoparticles either physically by adsorbing Tf or covalently conjugating Tf could be internalized by the ceils through clathrin-dependent and transferrin receptor-mediated endocytosis. Our study implicates that FND-PL-Tf nanoparticles have potential targeted drug delivery and tumor-targeting recognition functions.

关 键 词：荧光纳米钻石 聚赖氨酸 转铁蛋白 细胞吸收机制 

分 类 号：Q599[生物学—生物化学]