黑莓松散型胚性愈伤组织的诱导  

Study on the Induction Method of Blackberry Friable Embryogenic Callus

作　　者：苗博瑛[1] 刘艳军[2] 杨静慧[2] 

机构地区：[1]天津市林业果树研究所,天津300381 [2]天津农学院,天津300380

出　　处：《山西农业科学》2014年第3期209-212,216,共5页Journal of Shanxi Agricultural Sciences

基　　金：天津市重大科技专项(12ZCDZNC04800)

摘　　要：为了诱导出黑莓松散型胚性愈伤组织,试验采用黑莓试管苗叶片、叶柄和茎段为外植体,在含有不同2,4-D及分裂素的MS培养基上进行愈伤组织的初步诱导,从中筛选出松散型愈伤组织的最佳培养基配方;再将诱导出的黑莓松散型愈伤组织转移到含有不同激素种类和浓度的MS培养基上进行松散型胚性愈伤组织的诱导,从中筛选出最佳培养基配方.结果表明,在1/8 MS＋3 mg/L 2,4-D＋100 mg/L Vc＋ 10 g/L蔗糖的培养基上可以诱导出松散的黑莓愈伤组织;先在MS＋2mg/L2,4-D＋1 mg/L KT＋ 100 mg/L Vc＋ 30 g/L蔗糖的培养基上进行胚性愈伤的诱导继代2～3次,然后转移到MS＋ 0.5 mg/L 2,4-D＋2 mg/L KT＋ 100 mg/L Vc＋ 30 g/L蔗糖的培养基上继续培养并继代3～5次后即可诱导出黑莓的松散型胚性愈伤组织.试验结果将为采用黑莓体细胞诱变育种研究奠定基础.Tissue culture methods were developed for reproducible induction of friable embryogenic callus established from the leaf,petiole and stem explants of blackberry.The soft callus was obtained by leaf culture on MS medium containing 2,4-diehlorophenoxyaeetic acid （2,4-D） of different concentrations.The callus from leaves were transfered onto the MS medium containing several growth regulators and concentrations for induction of friable embryogenic callus.The results of experiments showed that the optical medium for induction of soft callus was 1/8 MS ＋ 3 mg/L 2,4-D ＋ 100 mg/L Vc ＋ 10 g/L sucrose; The methord of induction of friable embryogenic callus was first tansfering the soft callusonto the medium MS ＋ 2 mg/L 2,4-D ＋ 1 mg/L KT ＋ 100 mg/LVc ＋ 30 g/L sucrose,and after subcultured 2-3 times,then the callus were transfered onto the new medium MS ＋ 0.5 mg/L 2,4-D ＋ 2 mg/L KT ＋ 100 mg/L Vc ＋30 g/L sucrose.The friable embryogenic callus of blackberry was induced by 3-5 times subculture.The results will lay a foundation for the study on somatic mutation breeding by blackberry.

关 键 词：黑莓 胚性愈伤组织 外植体 体细胞诱变 激动素 

分 类 号：S663.2[农业科学—果树学]