重组白细胞介素-2高效纯化方法建立  

Establishing Efficient Purification Method of Recombinant Interleukin-2

作　　者：王坤[1] 李海涛[1] 张润祥[1] 朱言柱[1] 刘艳环[1] 冯卓[1] 苗利光[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《特产研究》2014年第1期30-34,共5页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技厅项目(20130102080JC)

摘　　要：依次通过离子交换法、凝胶过滤法纯化重组白细胞介素-2(IL-2)粗品,利用SDS-PAGE电泳和hIL-2 Elisa kit检测纯化后重组IL-2的纯度和浓度。结果表明,纯化后的重组IL-2纯度和浓度高。本纯化方法简单、稳定、纯化效果好,可为重组IL-2的生产工艺提供技术支持和依据。The sample of recombinant inlerleukin- 2wits purified by Ion exchange method and Gel filtration respectively. The purity and the concentration of RIL - 2 were determined by SDS - Page and hIL - 2 Elisa kit. The results showed that purity and the coneentration of RIL - 2 were high.This method is simple and stable, and the efficient purity of RIL - 2 can be obtained, it can provide evidence to produce RIL - 2 for industry.

关 键 词：重组白细胞介素-2 离子交换法 凝胶过滤法 纯化策略 

分 类 号：Q513.23[生物学—生物化学]