2009年河北省小麦叶锈菌群体EST-SSR遗传多样性分析  

Population Genetic Diversity of Puccinia triticina Based on EST-SSR in Hebei in 2009

作　　者：赵盼盼[1] 张林亚[1] 贾胜娟[1] 闫红飞[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院、河北省农作物病虫害生物防治工程技术研究中心、国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《西北农业学报》2014年第2期61-67,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家重点基础研究发展计划(2013CB127700);国家公益性行业(农业)科研专项(200903035);河北省留学人员科技活动项目择优资助项目(20120340);教育部博士点基金(2010130212005)

摘　　要：通过对小麦叶锈菌群体毒性多态性和EST-SSR多态性分析，为河北省小麦抗病品种的培育和合理利用提供依据。利用34个小麦抗叶锈近等（单）基因系和21对ESTSSR引物对河北省唐山、秦皇岛、沧州、保定、石家庄、邢台和邯郸的30个单孢子分离纯化小麦叶锈菌株进行毒性和DNA多态性分析。结果表明：30株小麦叶锈菌菌株间毒性相似性较高，相似系数为0．70～1．00，分子遗传多态性的相似系数为0．80～1．00。部分地理来源相同的菌株聚在同一亚组，表明小麦叶锈菌株地理来源与毒性多态性和遗传多态性有一定的相关性，但毒性多态性和SSR分子多态性相关性不明显。The molecular polymorphisms and virulence polymorphisms for Puccinia triticina will pro- vide the basis for breeding and rational utilization of disease-resistant varieties of wheat. Total 30 sin- gle uredinial isolates from Tangshan, Qinhuangdao, Cangzhou, Baoding, Shijiazhuang, Xingtai and Handan in Hebei province, were tested virulence with 34 near-isogenic lines containing known leaf rust （Lr） resistance genes, and evaluated genetic diversity with 21 pairs of EST-SSR primers. Den- drogram analysis showed that the similarity coefficients of 30 isolates were 0. 70-- 1. 00, indicating high virulence similarity among differential regions, the genetic similarity coefficients were 0. 80- 1.00, part of the same source were clustered in the same subgroup, which means the virulence poly- morphisms and molecular polymorphisms had some correlation with isolates origin. However, the correlation was weak between the virulence polymorphisms and the genetic polymorphisms based on EST-SSR.

关 键 词：小麦叶锈菌 EST—SSR 毒性 遗传多样性 

分 类 号：S435.121.43[农业科学—农业昆虫与害虫防治]