基因重组人睫状神经营养因子单克隆抗体的制备及初步鉴定  

Preparation and identification of monoclonal antibodies against recombinant human ciliary neurotrophil factor

作　　者：张亚莉[1] 苏瑾 车小燕[1] 林来兴妹[1] 周俊岭[2] 赵青[2] 王小宁[1] 

机构地区：[1]第一军医大学分子免疫学研究所,广州510515 [2]第一军医大学中心实验室,广州510515

出　　处：《第一军医大学学报》1999年第6期520-522,共3页Journal of First Military Medical University

摘　　要：目的　制备基因重组人睫状神经营养因子 (rhCNTF)单克隆抗体。方法利用rhCNTF免疫BALB/C小鼠 ,取其脾细胞与小鼠NS -1骨髓瘤细胞融合 ,制备 3株抗rhCNTF的单克隆抗体 ,分别命名为CA2 、CA6 和BB11。结果 3株抗体类型均为IgG2a,免疫印迹试验证明为特异性抗CNTF的单克隆抗体 ;抗体相加试验表明 3株单抗是针对CNTF两个不同的抗原决定簇。Objective To produce monoelonal antibodies against recombinant human ciliary neurotrophic factor (rhCNTF) Methods rhCNTF was used to immune BALB/C mouse, the spleen cells from which were fused with murine NS-1 myeloma cells 3 monoclonal antibodies against rhCNTF were produced from the hybridoma, named CA2, CA6 and BB 11 respectively Results All the 3 antibodies were in the IgG2a subclass and proved to be specific antibodies against CNTF By Western blot experiment, McAb additivity test showed that the 3 antibodies recognize 2 different epitopes

关 键 词：基因重组 人睫状神经营养因子 单克隆抗体 

分 类 号：R392－33[医药卫生—免疫学]