糖调节受损大鼠模型的建立与评价  

Establishment and Evaluation of Rat Models of Impaired Glucose Regulation

作　　者：王东[1] 李敬林[1] 

机构地区：[1]辽宁中医药大学附属医院内分泌科,辽宁沈阳10032

出　　处：《中华中医药学刊》2014年第3期463-464,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30973778)

摘　　要：目的:制备一种发病过程类似人类的糖调节受损大鼠模型。建立评价大鼠糖调节受损的诊断标准。方法:SPF级Wistar雄性大鼠高脂高糖饲料喂养12周,诱发大鼠胰岛素抵抗及胰岛β细胞损伤,建立大鼠糖调节受损模型。通过本实验制定的诊断标准筛选IGR大鼠模型,对筛选出的大鼠进行各项指标的测定以明确诊断标准的合理性。结果:筛选出的大鼠体重较对照组明显升高(P<0.05);空腹及OGTT 2h血糖较对照组有明显升高且存在显著差异(P<0.05);高糖钳夹实验胰岛素输注速率较对照组降低且存在显著差异(P<0.05);正糖钳夹实验第1时相、第2时相胰岛素分泌均较对照组低且存在显著差异(P<0.05)。结论:本实验糖调节受损大鼠模型造模成功,诊断标准具有科学性。本模型的建立简单易行,成模率高,是人类糖调节受损发病机制及干预手段研究的理想动物模型。Objective：To prepare a pathogenesis similar to human impaired glucose regulation in rat model. To estab- lish the evaluation in rats with impaired glucose regulation in diagnostic criteria. Methods：SPF Wistar male rats were fed with high - fat diet for 12 weeks and induced by insulin resistance and beta cell injury to establish a rat model of impaired glucose regulation. Through this experiment,it was to develop diagnostic criteria for screening of IGR rat model and to fil- ter out rat for measuring various indexes in order to clear the diagnosis standard of reasonableness. Results：The body weight of the screening out rats was higher than that of control group （P 〈 0.05）. OGTI＇ 2h fasting and glucose were sig- nificantly higher than tllose in the control group and there was significant difference （P 〈 0.05）. The high glucose clamp experiments the insulin infusion rate lower than control group and there was significant difference （P 〈 0.05）. In the first and the second phase of insulin glucose clamp experiments, the insulin secretions were lower than those in the controls and there was significant difference （ P 〈 O. 05 ）. Conclusion ： The results of impaired glucose regulation in rat model had a success, and the diagnostic criteria are scientific. This model is simple and easy with a high succussful rate. It is the ide- al animal model of impaired glucose regulation pathogenesis and intervention study.

关 键 词：糖调节受损 动物模型 胰岛功能 胰岛素抵抗 

分 类 号：R589[医药卫生—内分泌]