留兰香油主要成分香芹酮和柠檬烯的GC含量测定及薄层鉴别  

Determination of Main Components of Spearmint Oil by GC and Identification by TLC

作　　者：康艳蕾[1] 苏璇[1] 唐法娣[2] 王砚[2] 吴素香[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江大学,浙江杭州310053

出　　处：《中华中医药学刊》2014年第3期661-664,I0006,共5页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：建立留兰香油中的主要有效成分的测定方法。方法：采用Aglient6890N气相色谱仪，HP-5弹性石英毛细管柱（30m×0．323mm×0．25μm），氢火焰离子化检测器，气化室温度250℃，检测室温度280℃，进样分流比为20：1，程序升温。采用正十一烷为内标对照品溶液。采用薄层色谱法对其主要成分进行定性鉴别。结果：留兰香油中柠檬烯质量浓度在41．7～1042．5μg·mL-1（R=0．999）间，香芹酮质量浓度在102．8—2570μg·mL（R=0．999）间均呈良好的线性关系，平均回收率分别为101．7％，103．0％，RSD值为1．92％和1．59％。薄层色谱清晰，空白对照无干扰。结论：含量测定方法简便，准确，稳定性高，能有效测定留兰香油中有效成分的含量。薄层鉴别专属性强，可用于留兰香油的定性鉴别。Objective：To establish the determination method of main active components of spearmint oil. Methods： Aglient 6890N gas chromatograph was used with HP -5 elastic quartz capillary column （30 m×0.323mm×0.25μm） and hydrogen flame ionization detector. The gasification chamber temperature was 250 ℃ ; testing room temperature was 280 ℃ ; sample split ratio was 20 ： 1 ; temperature program control was used. N - hexane was selected as internal standard solution. TLC was used to identify the main active components of spearmint oil. Results ： For the GC quantitation method, the calibration curve of limonene of spearmint oil displayed ideal linearity in the range of 41.7 - 1042.5 μg mL-l. And the calibration curve of carvone displayed ideal linearity in the range of 102.8 - 2570 μg mL-1. The average recoveries were 101.7% , 103% and RSD values were 1.92% and 1.59%. The TLC figure was clear. The blank test showed no in- terference. Conclusion：The quantitative determination method was sensitive, reliable and repeatable, and could effectively detect the effective components of spearmint oil. TLC was specific and has less interference.

关 键 词：留兰香油 柠檬烯 香芹酮 气相色谱 薄层鉴别 

分 类 号：R284.1[医药卫生—中药学]