人胚胎外周组织褪黑素受体的鉴定  

Identification of melatonin receptor in human embryonic peripheral tissue

作　　者：刘志民[1] 邹俊杰[1] 沈玉美[1] 陆祖谦[1] 何淑芬[2] 彭树勋[2] 

机构地区：[1]第二军医大学长征医院内分泌科,上海200003 [2]香港大学医学院生理系

出　　处：《第二军医大学学报》2001年第1期8-11,共4页Academic Journal of Second Military Medical University

基　　金：香港裘槎基金资助项目 !(396 6 30 0 8)

摘　　要：目的 :探讨人胚胎外周组织褪黑素 (Mel)受体 (MR)体内分布的蛋白结合活性及信号转导途径。 方法 :应用放射配体结合法检测人胚胎外周组织 MR,并以 GTPγS观察其对 Mel特异结合的影响。 结果 :经饱和分析、动力学分析及特异性分析证明人胚胎心、肝、肾及甲状腺等 16种组织均存在 Mel特异结合位点 ,亚细胞分布研究表明这些组织中以细胞核及线粒体MR含量最高 ;GTPγS能够抑制 Mel与其受体的结合 ,使 MR的结合容量或亲和力降低。结论 :MR在人胚胎组织存在广泛 ,为解释 Mel作用的广泛性提供了实验依据。实验结果支持 MR是由抑制性Objective: To determine protein binding characteristic and signal transmission pathway of melatonin(Mel) receptor(MR) in human embryonic peripheral organ tissues. Methods: MR was measured by radio ligand binding assay and the effect of GTPγS on melatonin specific binding was studied. Results: Mel specific binding sites were determined in 16 kinds of human embryonic tissue and this binding could be inhibited by GTPγS, supporting the theory that MR is coupled to inhibitory G proteins system. Conclusion: MR is measured in human embryo tissue, these results provide experimental data for elucidating the mechanism of the effect of Mel.

关 键 词：褪黑素 受体 胚胎外周组织 MEL MR 

分 类 号：Q576[生物学—生物化学]