用原位杂交及免疫组化方法显示人胚胎甲状腺褪黑素受体  

Detecting melatonin receptor in thyroid of human embryo with immunohistochemistry and in situ hybridization

作　　者：孙中安[1] 陆祖谦[1] 刘志民[1] 

机构地区：[1]第二军医大学长征医院内分泌科,上海200003

出　　处：《第二军医大学学报》2001年第1期15-17,共3页Academic Journal of Second Military Medical University

基　　金：香港裘槎基金资助项目!(396 6 30 0 8)

摘　　要：目的 :在前期工作的基础上进一步观察人胚胎甲状腺组织中褪黑素 (Mel)受体的存在及其分布。方法 :取人胚胎 (胎龄 6个月 )甲状腺 ,石蜡包埋 ,切片 ,以免疫组化 ABC法及原位杂交方法进行染色。结果 :免疫组化方法显示 ,人胚胎甲状腺细胞的细胞膜、细胞质和细胞核中 Mel受体 m t1 和 MT2 亚型均呈棕褐色阳性染色 ;原位杂交方法显示 mt1 和 MT2 分布于甲状腺细胞的细胞质及细胞核内 ,呈蓝紫色阳性染色。 结论 :人胚胎甲状腺组织中存在褪黑素受体 mt1 和 MT2 亚型 ,分布于甲状腺细胞的细胞膜、细胞质。Objective: To make it clear whether there exists melatonin receptor in the thyroid of human embryo. Methods: Thyroid was collected and sliced up to be stained with methods of immunohistochemistry and in situ hybridization. Results: The thyroid tissue was positively dyed, melatonin receptor mt 1 and MT 2 were with both immunohistochemistry and in situ hybridization while brown granules deposited in the membrane, plasma and nuclear of the thyroid cell were with the immunohitochemistry. Conclusion: There exists melatonin receptors in human embryo thyroid, either mt 1 or MT 2, and they exist in the membrane, plasma and nuclear.

关 键 词：受体 褪黑素 甲状腺 原位杂交 免疫组织化学 胚胎 MEL MR 

分 类 号：Q576[生物学—生物化学]