小花琉璃草多糖提取工艺研究  

Cynoglossum lanceolatum Forsk Polysaccharide Extraction Technology

作　　者：缪天琳[1,2] 张跃华[1] 李文龙[1] 刘娟[2] 

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007 [2]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2013年第33期12841-12842,12845,共3页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省卫生厅课题(2012-268);佳木斯大学校重点课题(SZ2009-011)

摘　　要：[目的]优选小花琉璃草的多糖提取工艺。[方法]以小花琉璃草主茎粉末为原料,采用热水浸提法提取小花琉璃草多糖,选取提取温度、提取时间、料液比和提取次数4个影响因素进行单因素试验,通过L9(34)正交试验确定小花琉璃草多糖提取的最佳提取工艺;并用苯酚-硫酸测定多糖含量。[结果]提取次数对小花琉璃草多糖含量影响最大,其次依次为料液比、提取时间和提取温度。最佳提取工艺条件为:提取次数2次,料液比1∶30(g/ml),提取温度90℃,提取时间3 h。[结论]在最佳提取条件下,多糖提取率最高可达2.28%。[ Objective ] To optimize polysaceharide extraction technology of Cynoglossum lanceolatum. [ Method ] With main stem powder of Cynoglossum lanceolatum as raw material, hot water extraction method was adopted to extract polysaceharide from Cynoglossum lanceolaturn. Extraction temperature, time, solid - liquid ratio and times were selected to conduct single factor test, the optimum extraction technique was determined by L9 （34 ） orthogonal test ; The polysaccharide content was determined by phenol - sulfuric acid. [ Result ] The order of influencing factors is as follows： extraction times 〉 solid - liquid ratio 〉 extraction time and extraction temperature. The optimum extraction conditions are： extraction times 2, solid -liquid ratio 1：30 （g/ml）, extraction temperature 90 ℃, extraction time 3 h. [ Conclusion ] Under the optimum extraction conditions, polysaceharide extraction rate could up to 2.28%.

关 键 词：小花琉璃草(Cynoglossum lanceolatum Forsk) 多糖提取 工艺优化 

分 类 号：S567[农业科学—中草药栽培]