波棱素脂质体的制备工艺及处方优化研究  

Study on preparation process and formulation optimization of herpetin liposomes

作　　者：张鑫[1] 谭睿[2] 顾健[1] 何黎黎[1] 樊利娜[1] 南星梅 

机构地区：[1]西南民族大学民族医药研究院,四川成都610041 [2]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中国中药杂志》2014年第6期1007-1010,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81674168);四川省科学技术厅科技攻关项目(2011 FZ0053);四川省应用基础研究项目(2012JY0027)

摘　　要：波棱素是波棱瓜子中的木脂素分离出来的活性单体成分,其具有抗肝损伤、抑制乙型肝炎病毒复制的作用。该实验首次采用薄膜分散法成功制备了波棱素脂质体,以包封率为指标,对处方工艺进行优化。优化后的最佳膜材处方为波棱素-大豆磷脂-胆固醇2.44∶78.05∶19.51,选用0.5%普流尼克(F68)溶液50℃下进行水合脱膜,并水浴超声时间20 min,制备得到的波棱素脂质体平均包封率为(94.50±2.15)%,粒径(119.2±10.7)nm,达到试验预期,这将为后期的肝靶向给药系统的研究奠定基础。Herpetin （HPT） is an active monomer constituent isolated from lignanoid in seeds of Herpetospermum caudigerum. HPT shows inhibitory effects in hepatic injury and HBV-DNA and the replication. In the study, we successfully prepare herpetin liposomes by film dispersion method for the first time. The prescription process was optimized, with the entrapment efficiency as the index. According to the optimized prescription, the mass ratio of HPT： phospholipids： cholesterol was 2.44：78.05：19.51, the hydration and de-molding process was performed with 0.5% F68 solution at 50 ℃, and the water-bath ultrasonic time was 20 min. The HPT liposomes prepared by this method showed an average entrapment efficiency of （94.50±2.15）% and a particle size of （119.2±10.7） nm, which was consistent with the trial expectations and will lay a solid foundation for the hepatic targeting delivery system in future.

关 键 词：波棱素 脂质体 制备工艺 处方优化 

分 类 号：TQ461[化学工程—制药化工]