狗脊不同炮制品水煎液抗维甲酸致雄性大鼠骨质疏松症研究  

Study on decoction's effect of different processed rhizomes of Cibotium barometz on retinoic acid induced male rats osteoporosis

作　　者：徐钢[1,2,3] 孙娜[1,2,3] 赵敏杰[1,2,3] 鞠成国[1,2,3] 贾天柱[1,2,3] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药炮制工程技术研究中心,辽宁大连116600 [3]国家中医药管理局中药炮制重点研究室,辽宁大连116600

出　　处：《中国中药杂志》2014年第6期1011-1015,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973938)

摘　　要：该研究比较了生狗脊、酒狗脊、盐狗脊、烫狗脊和蒸狗脊的水煎液HPLC图谱,在此基础上,以砂烫骨碎补水煎液为阳性组,又比较了各组灌胃水煎液(质量浓度为104.2 g·L-1)4周后对维甲酸致骨质疏松雄性大鼠血清抗酒石酸酸性磷酸酶s-TRAP水平和作用机制指标血清护骨素OPG,血Ca和P,IL-6,TNF-α和IL-1水平及总评分的影响。结果表明,酒狗脊和砂烫狗脊效果较好,而蒸狗脊、盐狗脊以及生狗脊作用次之。在炮制方法的选择方面,加热方式中干热的砂烫法优于湿热的水蒸法,辅料中黄酒优于食盐。该结果为阐明狗脊炮制原理和抗骨质疏松症作用机制提供参考。This study compared the decoction＇s HPLC figures of the different processed rhizomes of Cibotium barometz including the raw, the sand-baked, the wined, the steamed and the salted, on the basis of which, with the sand-baked Drynaria fortunei decoction as the positive control group, comparingall groups＇decoction, concentration of which was 104.2 g·L^-1, for 4 weeks, by their effects （s-TRAP and total scores of OPG, Ca, P, IL-6, TNF-α and IL-1） on retinoic acid induced male rats osteoporosis. The experiment results showed the sand-baked and the wined were better than the steamed, the salted and the raw;in the processing methods＇ selection, the sand-baked was a better heating method than the steamed and the rice wine was the better excipient than the salt. It provided a reference to explain the processing principle of rhizomes of C. barometz and work mechanism of anti-osteoporosis.

关 键 词：狗脊 炮制品 水煎液 维甲酸 骨质疏松症 破骨细胞 

分 类 号：R285.5[医药卫生—中药学]